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PMID: 376870 Published · ppublish English Journal Article

Q beta-defective particles produced in a streptomycin-resistant Escherichia coli mutant.

Journal of virology ·Vol. 29 ·No. 3 ·1979-03-00 ·Pages 1107-17

Engelberg-Kulka H, Israeli-Reches M, Dekel L, Friedmann A

Abstract

This paper describes Q beta noninfectious particles produced at 41 degrees C in a streptomycin-resistant Escherichia coli mutant which is temperature sensitive for suppression of a nonsense codon. The noninfectious particles resembled Q beta under the electron microscope and contained coat protein molecules in an amount similar to the amount in Q beta. However, they did not adsorb to F-piliated bacteria, and they were deficient in both minor capsid proteins of Q beta, maturation (IIa) and read-through (IIb). Proteins IIa and IIb were not produced in Qbeta-infected mutant cells at 41 degrees C. In addition, instead of the 30S RNA of Q beta, a shorter RNA, which sedimented mainly at 23 S, was found in the defective particles. The results are discussed in relation to the roles of proteins IIa and IIb of Q beta.

MeSH Terms
Adsorption Coliphages/growth & development,ultrastructure Defective Viruses/growth & development,ultrastructure Drug Resistance, Microbial Escherichia coli/drug effects,genetics Mutation RNA, Viral/analysis Streptomycin/pharmacology Temperature Viral Proteins/analysis Virus Replication
Chemicals
RNA, Viral Viral Proteins Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Engelberg-Kulka H
Israeli-Reches M
Dekel L
Friedmann A
References (34)
34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.3.1107-1117.1979
Published
1979-03-00
Pages
1107-17
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353271
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.3.1107-1117.1979 PMC Full Text View journal details
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