Home LiteratureArticle Details
PMID: 377008 Published · ppublish English Journal Article

Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate.

Molecular & general genetics : MGG ·Vol. 168 ·No. 2 ·1979-01-10 ·Pages 141-51

Little JG, Haynes RH

Abstract

Mutant strains of Saccharomyces cerevisiae auxotrophic for deoxythymidine monophosphate (dTMP) were isolated and characterized. Two distinct classes of auxotrophs were obtained. One class had a simple requirement for dTMP and was analogous to thymine-requiring bacteria. The second class required dTMP, adenine, histidine and methionine and this complex nutritional phenotype was due to defects in folate metabolism. The dTMP-dependent growth of respiratory-competent grande auxotrophs was found to be markedly affected by media composition and carbon source. In the absence of dTMP thymineless death occurred in both mutant classes.

MeSH Terms
DNA Replication Folic Acid/genetics,metabolism Mutation Saccharomyces cerevisiae/genetics Thymidine Monophosphate/genetics,metabolism Thymine Nucleotides/genetics
Chemicals
Thymine Nucleotides Thymidine Monophosphate Folic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Little J G
Haynes R H
References (33)
33 references, click to expand
  1. Temperature-sensitive revertants.
    Biochem Biophys Res Commun. 1971 Dec 3;45(5):1337-43 PMID: 4944358
  2. A simple method for the isolation and characterization of thymidylate uptaking mutants in Saccharomyces cerevisiae.
    Mol Gen Genet. 1976 Aug 19;147(2):209-15 PMID: 787759
  3. Mutants of Saccharomyces able to grow after inhibition of thymidine phosphate synthesis.
    Mol Gen Genet. 1973 Sep 12;125(3):275-7 PMID: 4774595
  4. Isolation and characterization of mutants of Saccharomyces cerevisiae auxotrophic and conditionally auxotrophic for 5'-dTMP.
    Z Naturforsch C. 1974 Nov-Dec;29(11-12):733-8 PMID: 4281200
  5. Yeast cell-cycle mutant cdc21 is a temperature-sensitive thymidylate auxotroph.
    Mol Gen Genet. 1976 Aug 2;146(3):313-5 PMID: 794696
  6. Mutants for the specific labelling of DNA in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1973 Apr 11;299(4):681-5 PMID: 4575183
  7. Induction of cytoplasmic respiratory deficient mutants in yeast by the folic acid analogue, methotrexate. I. Studies on the mechanism of petite induction.
    Mol Gen Genet. 1973 Oct 16;126(1):61-70 PMID: 4591370
  8. Isolation and biochemical characterization of folate deficient mutants of Chinese hamster cells.
    Cell. 1974 Jul;2(3):173-82 PMID: 4547236
  9. The organization and replication of genetic material in yeast.
    Cell. 1975 Nov;6(3):259-68 PMID: 802680
  10. Isolation and properties of a thymidylate-less mutant in Saccharomyces cerevisiae.
    Eur J Biochem. 1975 Aug 15;56(2):533-8 PMID: 1100397
  11. Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Sep;115(3):966-74 PMID: 4580573
  12. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.
    J Gen Microbiol. 1968 Dec;54(2):307-17 PMID: 5729618
  13. Biogenetic autonomy of mitochondria.
    CRC Crit Rev Biochem. 1973 Aug;1(3):381-460 PMID: 4609690
  14. Induction of the cytoplasmic petite mutation in Saccharomyces cerevisiae by the antibacterial antibiotics erythromycin and chloramphenicol.
    Mol Gen Genet. 1971;111(3):209-23 PMID: 5563934
  15. Two forms of serine transhydroxymethylase, one absent in a thymidylate-less mutant in Saccharomyces cerevisiae.
    Eur J Biochem. 1976 Apr 15;64(1):7-13 PMID: 6271
  16. Thymidine 5'-monophosphate-requiring mutants of Saccharomyces cerevisiae are deficient in thymidylate synthetase.
    J Bacteriol. 1977 Oct;132(1):44-50 PMID: 334734
  17. Effect of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle.
    J Bacteriol. 1973 Jan;113(1):263-70 PMID: 4120066
  18. Interactions among genes controlling sensitivity to radiation and alkylation in yeast.
    Mol Gen Genet. 1973 Sep 12;125(3):197-216 PMID: 4359562
  19. New mutant types at the ade3 locus of Saccharomyces cerevisiae.
    J Bacteriol. 1971 Jul;107(1):1-7 PMID: 5563870
  20. N-formylmethionyl transfer RNA in mitochondria from yeast and rat liver.
    J Mol Biol. 1968 Dec 14;38(2):241-3 PMID: 5760639
  21. The identification of hexa-, hepta- and octoglutamates as the polyglutamyl forms of folate found throughout the growth cycle of yeast.
    J Gen Microbiol. 1976 Mar;93(1):169-72 PMID: 772164
  22. Mitochondrial and cytoplasmic distribution in Saccharmoyces cerevisiae of enzymes involved in folate-coenzyme-mediated one-carbon-group transfer. A genetic and biochemical study of the enzyme deficiencies in mutants tmp3 and ade3.
    Eur J Biochem. 1977 Sep 15;79(1):285-92 PMID: 334546
  23. Mutations affecting levels of tetrahydrofolate interconversion enzymes in Saccharomyces cerevisiae. I. Enzyme levels in ade3-41 and ADE15, a dominant adenine auxotroph.
    Mol Gen Genet. 1973 Jul 2;123(3):199-208 PMID: 4147243
  24. Mutants of Saccharomyces cerevisiae that incorporate deoxythymidine-5'-monophosphate into deoxyribonucleic acid in vivo.
    J Bacteriol. 1974 Jan;117(1):252-60 PMID: 4587606
  25. Economizing DNA-specific labelling by deoxythymidine-5'-monophosphate in Saccharomyces cerevisiae.
    Mol Gen Genet. 1974;132(4):335-45 PMID: 4610341
  26. Synthesis and breakdown of natural folates (folate polyglutamates).
    Prog Hematol. 1975;9:85-105 PMID: 766077
  27. Repair systems in Saccharomyces.
    Mutat Res. 1974 Aug;26(4):257-64 PMID: 4605044
  28. Genetic damage during thymidylate starvation in Saccharomyces cerevisiae.
    Mol Gen Genet. 1978 Mar 20;160(1):33-40 PMID: 347246
  29. Cellular and mitochondrial effects of folate antagonism by pyrimethamine in Saccharomyces cerevisiae.
    J Gen Microbiol. 1974 Aug;83(2):283-93 PMID: 4610084
  30. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1954 Oct;40(10):885-93 PMID: 16589586
  31. Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Feb;113(2):727-38 PMID: 4570606
  32. Synergistic interactions between rad mutations in yeast.
    Mutat Res. 1973 Oct;20(1):35-44 PMID: 4586553
  33. Mutants of formyltetrahydrofolate interconversion pathway of Saccharomyces cerevisiae.
    Genetics. 1977 May;86(1):85-102 PMID: 328341
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00431440
Published
1979-01-10
Pages
141-51
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00431440
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2