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PMID: 3770955 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Chromosomal map location of the alpha-hemolysin structural gene in Staphylococcus aureus NCTC 8325.

Infection and immunity ·Vol. 54 ·No. 2 ·1986-11-00 ·Pages 593-6

Pattee PA

Abstract

The alpha-hemolysin structural gene, hly+, previously cloned, insertionally inactivated, and introduced into the chromosome by allele replacement (M.O. O'Reilly, J.C.S. De Azavedo, S. Kennedy, and T.J. Foster, Microb. Pathog. 1:125-138, 1986), was shown by protoplast fusion and transformation to be in the gene order purC-hly-uraB-omega[chr::Tn916]1101-thrB on the chromosome of Staphylococcus aureus NCTC 8325. This location is clearly distinct from that of the agr determinant, a regulatory gene affecting several extracellular proteins, including alpha-hemolysin, located between tmn and ilv.

MeSH Terms
Alleles Bacterial Toxins/genetics Chromosome Mapping Chromosomes, Bacterial/physiology Cloning, Molecular Genes Genes, Bacterial Genotype Hemolysin Proteins/genetics Staphylococcus aureus/genetics Transformation, Bacterial
Chemicals
Bacterial Toxins Hemolysin Proteins staphylococcal alpha-toxin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pattee P A
References (17)
17 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.54.2.593-596.1986
Published
1986-11-00
Pages
593-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260204
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.54.2.593-596.1986 PMC Full Text View journal details
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