Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2222-6

Charnay P, Pourcel C, Louise A, Fritsch A, Tiollais P

Abstract

A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.

MeSH Terms

Chromosome Mapping Coliphages DNA Restriction Enzymes DNA, Recombinant DNA, Single-Stranded/genetics DNA, Viral/genetics Escherichia coli Hepatitis B virus/genetics

Chemicals

DNA, Recombinant DNA, Single-Stranded DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Charnay P

Pourcel C

Louise A

Fritsch A

Tiollais P

References (14)

14 references, click to expand

The genome of hepatitis B virus.
Annu Rev Microbiol. 1977;31:357-77 PMID: 334041
Structure of hepatitis B Dane particle DNA before and after the Dane particle DNA polymerase reaction.
J Virol. 1977 Feb;21(2):666-72 PMID: 833942
[Hepatitis B virus and primary liver carcinoma: evidences for a filiation hepatitis B, cirrhosis and primary liver cancer].
Ann Microbiol (Paris). 1977 Feb-Mar;128(2):245-53 PMID: 71006
Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction.
J Virol. 1977 Aug;23(2):368-76 PMID: 69727
Hepatitis B virus DNA in primary hepatocellular carcinoma tissue.
J Med Virol. 1978;2(3):207-14 PMID: 212525
[Cloning of the hepatitis B virus genome in Escherichia coli].
C R Acad Sci Hebd Seances Acad Sci D. 1978 Dec;287(16):1453-6 PMID: 158442
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.
Mol Gen Genet. 1979 Feb 26;170(2):171-8 PMID: 107393
Australia antigen and the biology of hepatitis B.
Science. 1977 Jul 1;197(4298):17-25 PMID: 325649
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Virus-like particles in serum of patients with Australia-antigen-associated hepatitis.
Lancet. 1970 Apr 4;1(7649):695-8 PMID: 4190997
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4597-601 PMID: 1060140
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA.
Nature. 1969 Sep 6;223(5210):1009-14 PMID: 5811898

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.5.2222

Published

1979-05-00

Pages

2222-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383570

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.5.2222 PMC Full Text View journal details

Analysis Services

Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.

Contact

WeChat Official Account