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PMID: 3777138 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detection of prolactin messenger RNA in rat anterior pituitary by in situ hybridization.

The American journal of pathology ·Vol. 125 ·No. 1 ·1986-10-00 ·Pages 35-44

Lloyd RV, Landefeld TD

Abstract

The effects of chronic diethylstilbestrol treatment on rat prolactin mRNA was analyzed by in situ hybridization histochemistry. Forty-day-old female rats were treated with 10 mg diethylstilbestrol in Silastic tubes for 3, 6, and 9 weeks. Estrogen treatment for 9 weeks increased pituitary wet weight (51.6 +/- 2.4 versus 7.9 +/- 0.31 mg for controls), serum prolactin (4155 +/- 571 versus 47.1 +/- 8.9 ng/ml for controls), and the percentage of immunoreactive prolactin cells (69% +/- 3% versus 34% +/- 2% for controls). In situ hybridization studies showed an increase in rat prolactin mRNA with increasing duration of estrogen treatment. After 9 weeks of estrogen treatment, there was a 2.3-fold increase in rat prolactin mRNA. 3H-cDNA was distributed diffusely throughout the anterior pituitary in both normal and hyperplastic pituitaries. There were no separate foci of adenomatous pituitary with increased labeling or with increased immunoreactive PRL cells. Although transplantable pituitary MtT/W15 tumors secreted very large amounts of PRL, compared with pituitaries from DES-treated rats, rat prolactin mRNA as evaluated by mean grain counts was considerably less in the MtT/W15 tumor than in DES-treated pituitary cells. These results show that in situ hybridization histochemistry can be used to detect changes in rat prolactin mRNA in tissue sections from the anterior pituitary with chronic estrogen treatment and that these pituitaries show a diffuse increase in immunoreactive prolactin cells and cellular prolactin mRNA, rather than distinct adenomatous areas within the glands.

MeSH Terms
Animals Diethylstilbestrol/pharmacology Female Growth Hormone/metabolism Histocytochemistry Immunochemistry Neoplasm Transplantation Nucleic Acid Hybridization Pituitary Gland, Anterior/metabolism Pituitary Neoplasms/genetics Prolactin/genetics,metabolism RNA, Messenger/metabolism Rats Rats, Inbred Strains
Chemicals
RNA, Messenger Diethylstilbestrol Prolactin Growth Hormone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lloyd R V
Landefeld T D
References (47)
47 references, click to expand
  1. Estrogen induced prolactin mRNA accumulation in adult male rat pituitary as revealed by in situ hybridization.
    Endocrinol Jpn. 1985 Oct;32(5):625-34 PMID: 4092669
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. Diethylstilbestrol inhibits tumor growth and prolactin production in rat pituitary tumors.
    Am J Pathol. 1985 Mar;118(3):379-86 PMID: 3976841
  4. Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis.
    Science. 1984 Aug 10;225(4662):632-4 PMID: 6740329
  5. The differential stimulatory effect of thyroid hormone on growth hormone synthesis and estrogen on prolactin synthesis due to accumulation of specific messenger ribonucleic acids.
    Endocrinology. 1979 Apr;104(4):1083-90 PMID: 436750
  6. Estrogen regulates the transcription of the rat prolactin gene in vivo through at least two independent mechanisms.
    Endocrinology. 1985 Jun;116(6):2456-62 PMID: 3996322
  7. Functional classification of cell types in the growth hormone- and prolactin-secreting rat MtTW mammosomatotropic tumor with ultrastructural immunocytochemistry.
    Am J Anat. 1980 Aug;158(4):455-61 PMID: 7006373
  8. Effect of estrogen on preprolactin messenger ribonucleic acid sequences.
    Biochemistry. 1979 May 15;18(10):2044-8 PMID: 435463
  9. Estrogen stimulates prolactin gene transcription by a mechanism independent of pituitary protein synthesis.
    Endocrinology. 1984 May;114(5):1550-7 PMID: 6201347
  10. Tissue-specific stimulation of RNA synthesis by transformed estradiol-receptor complex.
    Biochem Biophys Res Commun. 1972 Jan 31;46(2):661-7 PMID: 5057899
  11. In vivo effect of sex hormones on the in vitro synthesis of prolactin and growth hormone in normal and pituitary tumor-bearing rats.
    Endocrinology. 1969 Jun;84(6):1475-83 PMID: 4305502
  12. Hybridization histochemistry: use of recombinant DNA as a "homing probe" for tissue localization of specific mRNA populations.
    Endocrinology. 1981 Jan;108(1):353-6 PMID: 7007023
  13. Effects of 17 beta-estradiol and medroxyprogesterone acetate upon MtTW15 mammosomatotropic pituitary tumor growth and hormone production in male and female rats.
    Cancer Res. 1981 May;41(5):1772-7 PMID: 7214344
  14. Section thickness and grain count variation in tritium autoradiography.
    Stain Technol. 1971 May;46(3):131-5 PMID: 4935948
  15. Detection of viral sequences of low reiteration frequency by in situ hybridization.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6125-9 PMID: 216014
  16. Comparison of primary and secondary stimulation of male rats by estradiol in terms of prolactin synthesis and mRNA accumulation in the pituitary.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):824-8 PMID: 284404
  17. Estradiol regulates the transcription of the prolactin gene.
    J Biol Chem. 1982 Mar 10;257(5):2133-6 PMID: 7061411
  18. Cytological localization of chorionic gonadotropin alpha and placental lactogen mRNAs during development of the human placenta.
    J Cell Biol. 1982 Apr;93(1):190-8 PMID: 6896058
  19. Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.
    J Histochem Cytochem. 1986 Jan;34(1):39-43 PMID: 3510246
  20. Estrogen control of prolactin synthesis in vitro.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5946-9 PMID: 282615
  21. Rapid inactivation of ovalbumin messenger ribonucleic acid after acute withdrawal of estrogen.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2357-61 PMID: 4526211
  22. Effects of estradiol-17beta and pimozide on prolactin synthesis in male and female rats.
    Endocrinology. 1977 Jul;101(1):76-84 PMID: 862562
  23. Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.
    Nucleic Acids Res. 1979 Mar;6(3):915-30 PMID: 375200
  24. Pituitary adenomas in old Sprague-Dawley rats: a histologic, ultrastructural, and immunocytochemical study.
    J Natl Cancer Inst. 1984 Nov;73(5):1143-66 PMID: 6092768
  25. Effect of estradiol-17 beta on preprolactin messenger ribonucleic acid activity in the rat pituitary gland.
    Biochemistry. 1977 Nov 1;16(22):4915-21 PMID: 911801
  26. Prolactin-like immunoreactivity: localization in nerve terminals of rat hypothalamus.
    Science. 1977 May 20;196(4292):899-900 PMID: 323973
  27. Neoplastic responses and correlated plasma prolactin levels in diethylstilbestrol-treated ACI and Sprague-Dawley rats.
    Cancer Res. 1979 Mar;39(3):773-8 PMID: 427764
  28. Changes in hormone sensitivity of pituitary mammotropes during progression from normal to autonomous.
    Cancer Res. 1961 Aug;21:913-20 PMID: 13694116
  29. Hybridization histochemistry.
    Anal Biochem. 1985 Aug 15;149(1):1-28 PMID: 2416238
  30. Effect of estrogen on gene expression in the chick oviduct.
    J Biol Chem. 1976 Apr 10;251(7):1960-8 PMID: 773931
  31. Prolactin synthesis in primary cultures of pituitary cells: regulation by estradiol.
    Mol Cell Endocrinol. 1982 Mar;25(3):277-94 PMID: 7067928
  32. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  33. Prolactin mRNA exists in rat hypothalamus.
    Endocrinology. 1984 May;114(5):1947-9 PMID: 6714173
  34. Early effects of stilboestrol on growth hormone and prolactin secretion and on pituitary mitotic activity in the male rat.
    J Endocrinol. 1973 Aug;58(2):227-31 PMID: 4726322
  35. Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization.
    Brain Res. 1981 May 4;211(2):433-8 PMID: 7237132
  36. Influence of diethylstilbestrol treatment on prolactin cells of female ACI and Sprague-Dawley rats.
    Cancer Res. 1979 Mar;39(3):779-84 PMID: 427765
  37. Dependent and autonomous mammotropic pituitary tumors in rats; their somatotropic features.
    Cancer Res. 1956 Aug;16(7):608-16 PMID: 13356285
  38. In situ hybridization histochemistry: a technique for the study of gene expression in single cells.
    DNA. 1983;2(2):157-63 PMID: 6307624
  39. Detection of messenger RNAs of alpha-fetoprotein and albumin in a human hepatoma cell line by in situ hybridization.
    Cancer Res. 1985 Apr;45(4):1730-6 PMID: 2579734
  40. Estrogen-induced hyperplasia and neoplasia in the rat anterior pituitary gland. An immunohistochemical study.
    Am J Pathol. 1983 Nov;113(2):198-206 PMID: 6638150
  41. A comparison of estrogen effects on uterine and pituitary growth and prolactin synthesis in F344 and Holtzman rats.
    Endocrinology. 1981 Nov;109(5):1700-7 PMID: 7297500
  42. Local action of intrahypophyseal implants of estrogen as revealed by staining with peroxidase-labeled antibody.
    Am J Anat. 1970 May;128(1):1-19 PMID: 4193920
  43. A simple, rapid, and sensitive DNA assay procedure.
    Anal Biochem. 1980 Mar 1;102(2):344-52 PMID: 6158890
  44. Mechanism of anterior pituitary tumor induction by estrogen.
    Anat Rec. 1956 May;125(1):65-81 PMID: 13340236
  45. Genetic differences in estrogen-induced deoxyribonucleic acid synthesis in the rat pituitary: correlations with pituitary tumor susceptibility.
    Endocrinology. 1982 Oct;111(4):1140-9 PMID: 7117195
  46. Immunochemical isolation of prolactin messenger RNA.
    J Biol Chem. 1980 Feb 10;255(3):854-9 PMID: 7356664
  47. Quantitative analysis of in situ hybridization methods for the detection of actin gene expression.
    Nucleic Acids Res. 1985 Mar 11;13(5):1777-99 PMID: 3889842
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1986-10-00
Pages
35-44
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1888439
Subset
IM
Grants
NICHD NIH HHS · HD12016 · United States
NCI NIH HHS · IR23CA37238 · United States
External Links
PubMed source PMC Full Text View journal details
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