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PMID: 378977 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of temperature on translocation frequency of the Tn3 element.

Journal of bacteriology ·Vol. 139 ·No. 2 ·1979-08-00 ·Pages 515-9

Kretschmer PJ, Cohen SN

Abstract

The effect of temperature on the translocation frequency of the Tn3 element was investigated. The temperature optimum for translocation of Tn3 was in the range from 26 to 30 degrees C. At temperatures above 30 degrees C, the translocation frequency decreased rapidly and linearly; at 36 degrees C it was only 5% of the frequency observed at 30 degrees C. The duration and reversibility of the temperature effect were utilized to demonstrate a requirement for protein synthesis in the translocation process.

MeSH Terms
Bacterial Proteins/biosynthesis Chloramphenicol/pharmacology DNA, Bacterial/genetics Escherichia coli/genetics Plasmids Recombination, Genetic Temperature
Chemicals
Bacterial Proteins DNA, Bacterial Chloramphenicol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kretschmer P J
Cohen S N
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.2.515-519.1979
Published
1979-08-00
Pages
515-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216898
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.2.515-519.1979 PMC Full Text View journal details
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