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PMID: 3796607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human proto-oncogene N-myc encodes nuclear proteins that bind DNA.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4450-7

Ramsay G, Stanton L, Schwab M, Bishop JM

Abstract

N-myc is a gene whose amplification has been implicated in the genesis of several malignant human tumors. We have identified two proteins with molecular weights of 65,000 and 67,000 encoded by N-myc. The abundance of these proteins in tumor cells was consonant with the extent of amplification of N-myc. The two proteins apparently arose from the same mRNA, were phosphorylated, were exceptionally unstable, were located in the nucleus of cells, and bound to both single- and double-stranded DNA. These properties suggest that the products of N-myc and of the related proto-oncogene c-myc may have similar biochemical functions and that N-myc may be a regulatory gene. Our findings sustain the view that inordinate expression of N-myc may contribute to the genesis of several different human tumors.

MeSH Terms
Apudoma Cell Line Cellulose/analogs & derivatives Chromatography, Affinity Colonic Neoplasms DNA/analogs & derivatives DNA-Binding Proteins/genetics,isolation & purification Genes HeLa Cells/metabolism Humans Neuroblastoma Nucleoproteins/genetics,isolation & purification Proto-Oncogene Mas Proto-Oncogenes
Chemicals
DNA-Binding Proteins DNA-cellulose MAS1 protein, human Nucleoproteins Proto-Oncogene Mas Cellulose DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ramsay G
Stanton L
Schwab M
Bishop J M
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4450-4457.1986
Published
1986-12-00
Pages
4450-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367228
Subset
IM
Grants
NCI NIH HHS · CA-12705 · United States
NCRR NIH HHS · SO7-RR05355 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4450-4457.1986 PMC Full Text View journal details
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