Home LiteratureArticle Details
PMID: 6513926 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2486-97

Hann SR, Eisenman RN

Abstract

To examine myc protein products in the wide variety of human tumor cells having alterations of the c-myc locus, we have prepared an antiserum against a synthetic peptide corresponding to the predicted C-terminal sequence of the human c-myc protein. This antiserum (anti-hu-myc 12C) specifically precipitated two proteins of 64 and 67 kilodaltons in quantities ranging from low levels in normal fibroblasts to 10-fold-higher levels in Epstein-Barr virus-immortalized and Burkitt's lymphoma cell lines, to 20- to 60-fold-higher levels in cell lines having amplified c-myc. The p64 and p67 proteins were found to be highly related by partial V8 proteolytic mapping, and both were demonstrated to be encoded by the c-myc oncogene, using hybrid-selected translation of myc-specific RNA. In addition, the p64 protein was specifically precipitated from cells transfected with a translocated c-myc gene. Both p64 and p67 were found to be nuclear phosphoproteins with extremely short half-lives. In tumor cell lines having alterations at the c-myc locus due to amplification or translocation, we observed a significant change in the expression of p64 relative to p67 when compared with normal or Epstein-Bar virus-immortalized cells.

MeSH Terms
Animals Cell Line Cell Nucleus/metabolism Chromosome Mapping Gene Expression Regulation Humans Mice Neoplasm Proteins/biosynthesis,genetics Neoplasms/genetics,metabolism Oncogenes Protein Biosynthesis Transfection
Chemicals
Neoplasm Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hann S R
Eisenman R N
References (48)
48 references, click to expand
  1. Identification of nuclear proteins encoded by viral and cellular myc oncogenes.
    Nature. 1983 Nov 17-23;306(5940):274-7 PMID: 6316149
  2. Defectiveness of avian myelocytomatosis virus MC29: isolation of long-term nonproducer cultures and analysis of virus-specific polypeptide synthesis.
    Virology. 1977 Oct 15;82(2):431-48 PMID: 199997
  3. Paradox and paradigm: the message and meaning of myc.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):733-6 PMID: 6606785
  4. Altered nucleotide sequences of a translocated c-myc gene in Burkitt lymphoma.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):760-5 PMID: 6419122
  5. Transcription enhancer identified near the human C mu immunoglobulin heavy chain gene is unavailable to the translocated c-myc gene in a Burkitt lymphoma.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):806-9 PMID: 6419124
  6. Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation.
    Cell. 1984 Feb;36(2):241-7 PMID: 6692471
  7. Activation and somatic mutation of the translocated c-myc gene in burkitt lymphoma cells.
    Cell. 1984 Feb;36(2):339-48 PMID: 6319017
  8. Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy-chain locus.
    Nature. 1984 Jan 26-Feb 1;307(5949):334-40 PMID: 6420706
  9. Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
    EMBO J. 1983;2(12):2375-83 PMID: 6321164
  10. Complete transformation by adenovirus 2 requires both E1A proteins.
    Cell. 1984 Apr;36(4):951-61 PMID: 6705049
  11. From c-fos to v-fos.
    Nature. 1984 Mar 22-28;308(5957):317 PMID: 6709039
  12. Alternative transcription and two modes of splicing results in two myosin light chains from one gene.
    Nature. 1984 Mar 22-28;308(5957):333-8 PMID: 6709041
  13. Activation of the c-myc gene by translocation: a model for translational control.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7476-80 PMID: 6324175
  14. Expression of cellular oncogenes in human malignancies.
    Science. 1984 Apr 20;224(4646):256-62 PMID: 6538699
  15. Common features of the yes and src gene products defined by peptide-specific antibodies.
    J Virol. 1984 Aug;51(2):539-46 PMID: 6205173
  16. Different structural systems of the nucleus are targets for SV40 large T antigen.
    Cell. 1983 May;33(1):173-81 PMID: 6088054
  17. The origin and destiny of adenovirus proteins.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:581-90 PMID: 1057484
  18. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  19. Transformation parameters in chicken fibroblasts transformed by AEV and MC29 avian leukemia viruses.
    Cell. 1978 Apr;13(4):751-60 PMID: 207439
  20. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  21. Coding potential and regulatory signals of the polyoma virus genome.
    Nature. 1980 Jan 31;283(5746):445-53 PMID: 6243401
  22. Three new types of viral oncogenes in defective avian leukemia viruses. I. Specific nucleotide sequences of cellular origin correlate with specific transformation.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1215-23 PMID: 6253195
  23. Genetic structure of avian acute leukemia viruses.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:801-22 PMID: 6253214
  24. Synthesis and processing of polymerase proteins of wild-type and mutant avian retroviruses.
    J Virol. 1980 Oct;36(1):62-78 PMID: 6160263
  25. Considerations in the isolation of rat liver nuclear matrix, nuclear envelope, and pore complex lamina.
    Exp Cell Res. 1981 Mar;132(1):105-23 PMID: 7202557
  26. Adenovirus type 2 early proteins: assignment of the early region 1A proteins synthesized in vivo and in vitro to specific mRNAs.
    Virology. 1981 Jul 30;112(2):703-13 PMID: 7257187
  27. Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region.
    Nucleic Acids Res. 1981 Aug 25;9(16):4023-42 PMID: 6985482
  28. Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells.
    Nature. 1982 Jan 14;295(5845):116-9 PMID: 6173755
  29. Expression of cellular homologues of retroviral onc genes in human hematopoietic cells.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2490-4 PMID: 6283530
  30. Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell line.
    Nature. 1982 Aug 12;298(5875):679-81 PMID: 6285209
  31. Onc gene amplification in promyelocytic leukaemia cell line HL-60 and primary leukaemic cells of the same patient.
    Nature. 1982 Sep 2;299(5878):61-3 PMID: 6955596
  32. Nuclear location of the putative transforming protein of avian myelocytomatosis virus.
    Cell. 1982 Jun;29(2):427-39 PMID: 6288259
  33. Nucleotide sequences from the adenovirus-2 genome.
    J Biol Chem. 1982 Nov 25;257(22):13475-91 PMID: 7142161
  34. Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29.
    Nature. 1983 Feb 24;301(5902):722-5 PMID: 6298632
  35. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707-11 PMID: 6300869
  36. Nucleotide sequence of cloned cDNA of human c-myc oncogene.
    Nature. 1983 Jun 23-29;303(5919):725-8 PMID: 6304538
  37. Nucleotide sequence analysis of human c-myc locus, chicken homologue, and myelocytomatosis virus MC29 transforming gene reveals a highly conserved gene product.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3642-5 PMID: 6304729
  38. Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells.
    Mol Cell Biol. 1983 May;3(5):829-38 PMID: 6346057
  39. Translocation joins c-myc and immunoglobulin gamma 1 genes in a Burkitt lymphoma revealing a third exon in the c-myc oncogene.
    Nature. 1983 Jul 14-20;304(5922):135-9 PMID: 6306472
  40. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
    Nature. 1983 Aug 18-24;304(5927):596-602 PMID: 6308472
  41. Differential expression of the normal and of the translocated human c-myc oncogenes in B cells.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4822-6 PMID: 6308654
  42. Antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity.
    J Biol Chem. 1983 Sep 25;258(18):11219-28 PMID: 6411728
  43. On the nature of crossreactions observed with antibodies directed to defined epitopes.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5939-43 PMID: 6193510
  44. Identification of the c-myc oncogene product in normal and malignant B cells.
    Science. 1983 Oct 28;222(4622):430-2 PMID: 6604943
  45. Proteins encoded by v-myc and c-myc oncogenes: identification and localization in acute leukemia virus transformants and bursal lymphoma cell lines.
    Cell. 1983 Oct;34(3):789-98 PMID: 6313208
  46. Amplification and expression of the c-myc oncogene in human lung cancer cell lines.
    Nature. 1983 Nov 10-16;306(5939):194-6 PMID: 6646201
  47. Intracellular protein degradation in growing, in density-inhibited, and in serum-restricted fibroblast cultures.
    J Cell Physiol. 1977 Sep;92(3):353-64 PMID: 198415
  48. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2486-2497.1984
Published
1984-11-00
Pages
2486-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369080
Subset
IM
Grants
NCI NIH HHS · CA 28151 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.11.2486-2497.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2