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PMID: 379817 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

Nucleic acids research ·Vol. 6 ·No. 6 ·1979-00-00 ·Pages 2133-50

Berg PE, Lewin A, Christianson T, Rabinowitz M

Abstract

Some of the EcoRI fragments of yeast (Saccharomyces cerevisiae) mitochondrial DNA were cloned into E. coli using plasmid pMB9. The five smallest fragments in molecular weight appeared to be preferentially retained by E coli; partial fragments derived from larger mitochondrial DNA fragments were also found. One of the fragments, R7 (2.4 kb), may contain the OII gene. Cloned R7 DNA was stable under a variety of growth conditions, but showed some changes in molecular weight after transfer to different E. coli strains. Fragment R7 is transcribed in minicells, producing RNA that hybridizes specifically to mitochondrial DNA. Both DNA strands are transcribed, in contrast to the asymmetric transcription found in mitochondria. No new polypeptides were observed in minicells containing cloned fragment 7.

MeSH Terms
DNA Restriction Enzymes DNA, Mitochondrial/metabolism DNA, Recombinant/metabolism Drug Stability Escherichia coli/metabolism Genes Molecular Weight Nucleic Acid Hybridization Plasmids RNA Saccharomyces cerevisiae/metabolism
Chemicals
DNA, Mitochondrial DNA, Recombinant RNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Berg P E
Lewin A
Christianson T
Rabinowitz M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.6.2133
Published
1979-00-00
Pages
2133-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327841
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.6.2133 PMC Full Text View journal details
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