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PMID: 379824 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction.

Nucleic acids research ·Vol. 6 ·No. 7 ·1979-06-11 ·Pages 2583-99

Sista HS, Loder RT, Caruthers MH

Abstract

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA) . poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the 5 methyl group are quite critical. For example a seven fold reduction in stability was observed for the transversion. Results also suggest that the operator spans at least 21 base pairs.

MeSH Terms
Adenine Base Sequence DNA Escherichia coli/analysis Genes Lac Operon Mutation Nucleic Acid Conformation Oligodeoxyribonucleotides/analysis Poly dA-dT Repressor Proteins Thymine Transcription Factors
Chemicals
Oligodeoxyribonucleotides Repressor Proteins Transcription Factors Poly dA-dT DNA Adenine Thymine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sista H S
Loder R T
Caruthers M H
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.7.2583
Published
1979-06-11
Pages
2583-99
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327873
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.7.2583 PMC Full Text View journal details
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