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PMID: 381171 Published · ppublish English Journal Article

B-cell precursors in early chicken embryos.

Immunology ·Vol. 37 ·No. 1 ·1979-05-00 ·Pages 123-34

Lebacq AM, Ritter MA

Abstract

The ontogeny of B-cell precursors in chicken embryos from day 3 of incubation onwards has been studied. Purified antibodies to chicken Ig L, gamma, mu, alpha chains were used in a sensitive indirect immunofluorescence assayed on fixed cell smears and wax-embedded tissue sections; the location and morphology of immunoglobulin positive (Ig+) cells were determined either in phase contrast or after histological staining. Lymphoid cells containing small amounts of cytoplasmic immunoglobulin were found in 3 day and older embryonic yolk sac, 11 and 12 day blood, 11, 12 and 13 day bursal mesenchyme. cIg+ large basophilic cells were first seen in 14 day bursal follicles. It is concluded that cells enter the embryonic bursa at different developmental stages: some appear to be uncommitted stem cells, whilst others have already commenced B-cell maturation in an extra-bursal site.

MeSH Terms
Animals B-Lymphocytes/cytology,immunology Bursa of Fabricius/cytology,immunology Chick Embryo/immunology Fluorescent Antibody Technique Immunoglobulin A/analysis Immunoglobulin G/analysis Immunoglobulin M/analysis Immunoglobulins/analysis Time Factors Yolk Sac/cytology,immunology
Chemicals
Immunoglobulin A Immunoglobulin G Immunoglobulin M Immunoglobulins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lebacq A M
Ritter M A
References (34)
34 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1979-05-00
Pages
123-34
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1457309
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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