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PMID: 381841 Published · ppublish English Journal Article

Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.

Molecular & general genetics : MGG ·Vol. 173 ·No. 1 ·1979-05-23 ·Pages 39-50

Fiil NP, Bendiak D, Collins J, Friesen JD

Abstract

Fragments of lambda drifd 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (RPLK), Li (rplA), L10 (rplJ) and L12 (rplL) as well as the beta (rpoB) ANd beta' (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two.

MeSH Terms
DNA, Recombinant DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Genes Genetic Linkage Operon Phenotype Plasmids Recombination, Genetic Ribosomal Proteins/genetics
Chemicals
DNA, Recombinant Ribosomal Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fiil N P
Bendiak D
Collins J
Friesen J D
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267689
Published
1979-05-23
Pages
39-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267689
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