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PMID: 3821734 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells.

Molecular and cellular biology ·Vol. 7 ·No. 2 ·1987-02-00 ·Pages 951-5

Klarsfeld A, Daubas P, Bourachot B, Changeux JP

Abstract

The 5' end and promoter region of the alpha-subunit gene of chicken muscle acetylcholine receptor was mapped and sequenced. It includes a TATA and a CAAT box and a potential Sp1-binding site. When inserted in front of the chloramphenicol acetyltransferase gene, this promoter (including 850 base pairs of upstream sequence) directed high transient chloramphenicol acetyltransferase expression in transfected mouse C2.7 myotubes but not in C2.7 myoblasts or nonmyogenic 3T6 cells.

MeSH Terms
Age Factors Animals Chickens Gene Expression Regulation Genes, Regulator Macromolecular Substances Mice Muscles/physiology Promoter Regions, Genetic Receptors, Nicotinic/genetics Species Specificity Tissue Distribution Transcription, Genetic Transfection
Chemicals
Macromolecular Substances Receptors, Nicotinic
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Klarsfeld A
Daubas P
Bourachot B
Changeux J P
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.2.951-955.1987
Published
1987-02-00
Pages
951-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365157
Subset
IM
Databases
GENBANK
M15307
External Links
PubMed source DOI: 10.1128/mcb.7.2.951-955.1987 PMC Full Text View journal details
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