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PMID: 6328484 Published · ppublish English Journal Article

Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 10 ·1984-05-00 ·Pages 2980-4

Seiler-Tuyns A, Eldridge JD, Paterson BM

Abstract

We have introduced the chicken genes for cytoplasmic beta-actin, cardiac alpha-actin, and skeletal alpha-actin into C2 cells, a murine myogenic cell line, and into L cells by using the simian virus 40-derived vector PSV2 -gpt. In each selection, the entire population of transformed cells was analyzed for the expression and regulation of the actin genes by nuclease S1 assay and primer extension. This was compared to the expression of the vector marker Eco-gpt. The beta-actin gene is transcribed accurately and efficiently both in L-cells and in undifferentiated C2 cells. In fused C2 cells, beta-actin transcripts decrease significantly in parallel with the endogenous level of mouse beta-actin mRNA. Eco-gpt RNA levels remain essentially constant during myogenesis. The alpha-actin genes are correctly expressed at low levels in L cells but at significantly higher levels in the C2 cell background. Unlike the endogenous mouse alpha-actin gene, this level of expression does not change measurably with myogenesis. The skeletal alpha-actin gene is expressed poorly in pre- and post-fusion C2 cells, displaying no induction with differentiation. These results suggest that the tissue specificity of expression is maintained but the pattern of gene regulation for the sarcomeric actins is not. Factors in addition to the sequences flanking these genes are important for modulating gene expression during development. The decrease in the levels of beta-actin RNA during C2 cell differentiation provides a model system in which to study gene repression during development.

MeSH Terms
Actins/genetics Animals Base Sequence Cell Line Chickens Cloning, Molecular DNA Restriction Enzymes Genes L Cells/metabolism Mice Muscles/metabolism Plasmids Transfection
Chemicals
Actins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Seiler-Tuyns A
Eldridge J D
Paterson B M
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.10.2980
Published
1984-05-00
Pages
2980-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345204
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.10.2980 PMC Full Text View journal details
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