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PMID: 384151 Published · ppublish English Journal Article

Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti.

Molecular & general genetics : MGG ·Vol. 173 ·No. 3 ·1979-06-20 ·Pages 289-98

Julliot JS, Boistard P

Abstract

RP4-prime plasmids containing chromosomal fragments of either Escherichia coli or Rhizobium meliloti were constructed in vitro. When introduced into E. coli or R. meliloti respectively, they promoted a polarized transfer of the chromosome as demonstrated either by the gradient of transfer of various markers or by the study of the genetic constitution of recombinants. In E. coli, mobilization was shown to be dependent upon the presence of a functional rec A system. Inheritance of markers was due to their integration into the chromosome of the recipient as shown by the need for a functional rec A system in the recipient E. coli or by mobilization of recessive markers in R. meliloti. The system described could be applied to genetic mapping in any Gram negative bacteria.

MeSH Terms
Bacteriological Techniques Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics Genetic Techniques Plasmids Rhizobium/genetics Transduction, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Julliot J S
Boistard P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268639
Published
1979-06-20
Pages
289-98
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268639
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