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PMID: 3857213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regional chromosome mapping of human collagen genes alpha 2(I) and alpha 1(I) (COLIA2 and COLIA1).

Human genetics ·Vol. 69 ·No. 4 ·1985-00-00 ·Pages 304-8

Retief E, Parker MI, Retief AE

Abstract

For the assignment of the genes for the pro-alpha 2(I) (COLIA2) and the pro-alpha 1(I) (COLIA1) collagens, cDNA and genomic DNA probes were used in in situ hybridization experiments on human prometaphase chromosomes. An improved staining method is reported for the simultaneous identification of chromosomes and the autoradiographic grains after the hybridization procedures. With this procedure more cells with higher resolution could be used for the assignment of genes by in situ hybridization. Statistical analysis of the grains located on respectively 660 and 302 metaphases using pro-alpha 2(I) and pro alpha 1(I) DNA probes, confirmed the assignment of these genes to human chromosomes 7 and 17. Analysis of the grain distribution on prometaphase chromosomes showed that the location of the pro-alpha 2(I) collagen gene is in the region 7q21.3-22.1. The location of the pro-alpha 1(I) collagen gene was found to be in band 17q21.31-2205.

MeSH Terms
Autoradiography Chromosome Banding Chromosome Mapping Chromosomes, Human, 16-18 Chromosomes, Human, 6-12 and X DNA/genetics Genes Genetic Markers Humans Nucleic Acid Hybridization Procollagen/genetics
Chemicals
Genetic Markers Procollagen DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Retief E
Parker M I
Retief A E
References (16)
16 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00291646
Published
1985-00-00
Pages
304-8
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00291646 View journal details
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