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PMID: 3858829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3405-9

Bell JI, Estess P, St John T, Saiki R, Watling DL, Erlich HA, McDevitt HO

Abstract

Two HLA class II beta-chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DR beta chain (2918.4) and a DQ beta chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DR alpha chain. The translation products were immunoprecipitated with a beta-chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for beta and alpha chains. Sequence comparisons of 2918.4 with previously published DR beta-chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQ beta chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQ beta sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.

MeSH Terms
Animals Base Sequence DNA/genetics Female Genes HLA-DR Antigens Histocompatibility Antigens Class II/genetics Isoelectric Point Major Histocompatibility Complex Molecular Weight Oocytes/physiology Xenopus laevis/physiology
Chemicals
HLA-DR Antigens Histocompatibility Antigens Class II DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bell J I
Estess P
St John T
Saiki R
Watling D L
Erlich H A
McDevitt H O
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3405
Published
1985-05-00
Pages
3405-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397784
Subset
IM
Grants
NIAID NIH HHS · AI 18367 · United States
Databases
GENBANK
M11161
External Links
PubMed source DOI: 10.1073/pnas.82.10.3405 PMC Full Text View journal details
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