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PMID: 386038 Published · ppublish English Journal Article

Deletions, insertions and rearrangements affecting rpoB gene expression.

Molecular & general genetics : MGG ·Vol. 173 ·No. 2 ·1979-06-07 ·Pages 217-20

Collins J

Abstract

Through cloning and deletion experiments on ColEl hybrids the rpoB gene (Rifr) was located on a physical restriction map; RNA polymerase binding studies showed no binding site within the structural gene. The genetic data and RNA polymerase binding studies lead to the conclusion that rp/L and rpoB are dependent upon a common promoter.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Genes Genetic Linkage Plasmids RNA Polymerase II/genetics Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Ribosomal Proteins RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Collins J
References (9)
9 references, click to expand
  1. Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.
    J Bacteriol. 1977 Oct;132(1):332-40 PMID: 334729
  2. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  3. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242-6 PMID: 360212
  4. Interaction of RNA polymerase with promoters from bacteriophage fd.
    Eur J Biochem. 1977 Mar 15;74(1):107-13 PMID: 300680
  5. Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3891-5 PMID: 358203
  6. Separation and characterization of the subunits of ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6168-76 PMID: 4900511
  7. The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR 322.
    Mol Gen Genet. 1978 Jul 25;163(3):301-5 PMID: 355856
  8. Identification of a single promoter in E. coli for rplJ, rplL and rpoBC.
    Nature. 1978 Nov 2;276(5683):33-7 PMID: 368640
  9. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.
    J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1979-06-07
Pages
217-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
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