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PMID: 3862122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Efficient insertion of genes into the mouse germ line via retroviral vectors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6148-52

van der Putten H, Botteri FM, Miller AD, Rosenfeld MG, Fan H, Evans RM, Verma IM

Abstract

We present a general strategy for the efficient insertion of recombinant retroviral vector DNA into the mouse germ line via infection of preimplantation mouse embryos. Transgenic mice were generated that harbor a replication-competent recombinant retrovirus (delta Mo + Py M-MuLV) that lacks the Moloney murine leukemia virus (M-MuLV)-type enhancer sequence in the long terminal repeat (LTR). Instead, the LTR contains an enhancer element that permits polyoma virus F101 to grow in undifferentiated F9 embryonal carcinoma cells. Expression studies in different tissues of animals transgenic for delta Mo + Py M-MuLV indicate possibilities to target and modulate expression of retroviral recombinants in mice via their LTR enhancer sequences. In addition, 16 transgenic mice were generated that harbor proviral DNA of a defective recombinant retrovirus carrying a mutant dihydrofolate reductase gene.

MeSH Terms
Animals DNA Replication DNA, Recombinant DNA, Viral/genetics Defective Viruses/genetics Embryo Transfer Embryo, Mammalian/physiology Embryonic Development Female Gene Expression Regulation Genetic Engineering/methods Mice Pregnancy Retroviridae/genetics Tetrahydrofolate Dehydrogenase/genetics
Chemicals
DNA, Recombinant DNA, Viral Tetrahydrofolate Dehydrogenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van der Putten H
Botteri F M
Miller A D
Rosenfeld M G
Fan H
Evans R M
Verma I M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6148
Published
1985-09-00
Pages
6148-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391009
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.18.6148 PMC Full Text View journal details
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