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PMID: 3865203 Published · ppublish English Journal Article

Cloning and expression of cDNA for rat heme oxygenase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 7865-9

Shibahara S, Müller R, Taguchi H, Yoshida T

Abstract

Two cDNA clones for rat heme oxygenase have been isolated from a rat spleen cDNA library in lambda gt11 by immunological screening using a specific polyclonal antibody. One of these clones has an insert of 1530 nucleotides that contains the entire protein-coding region. To confirm that the isolated cDNA encodes heme oxygenase, we transfected monkey kidney cells (COS-7) with the cDNA carried in a simian virus 40 vector. The heme oxygenase was highly expressed in endoplasmic reticulum of transfected cells. The nucleotide sequence of the cloned cDNA was determined and the primary structure of heme oxygenase was deduced. Heme oxygenase is composed of 289 amino acids and has one hydrophobic segment at its carboxyl terminus, which is probably important for the insertion of heme oxygenase into endoplasmic reticulum. The cloned cDNA was used to analyze the induction of heme oxygenase in rat liver by treatment with CoCl2 or with hemin. RNA blot analysis showed that both CoCl2 and hemin increased the amount of hybridizable mRNA, suggesting that these substances may act at the transcriptional level to increase the amount of heme oxygenase.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Chlorocebus aethiops Cloning, Molecular DNA/genetics Gene Expression Regulation Heme Oxygenase (Decyclizing)/genetics,immunology Mixed Function Oxygenases/genetics Protein Conformation RNA, Messenger/genetics Rats Solubility
Chemicals
RNA, Messenger DNA Mixed Function Oxygenases Heme Oxygenase (Decyclizing)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shibahara S
Müller R
Taguchi H
Yoshida T
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.7865
Published
1985-12-00
Pages
7865-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390870
Subset
IM
Databases
GENBANK
J02722, M12129
External Links
PubMed source DOI: 10.1073/pnas.82.23.7865 PMC Full Text View journal details
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