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PMID: 3865896 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

DNA sequence analysis of a rat RT1 class II A beta gene.

Immunogenetics ·Vol. 22 ·No. 6 ·1985-00-00 ·Pages 653-63

Eccles SJ, McMaster WR

Abstract

The rat major histocompatibility complex (RT1) encodes twin sets of class II molecules, each consisting of two polypeptide chains referred to as A alpha and A beta, and E alpha and E beta. A gene encoding the RT1.A beta chain was isolated from a rat genomic library using an HLA-DQ beta chain cDNA as a probe. The nucleotide sequence of the coding regions of this gene was determined. Comparison of this sequence with those of the corresponding genes of mouse (H-2A beta) and human (HLA-DQ beta) revealed that this gene has been highly conserved during evolution, and that some parts of the molecule are more conserved than others. Analysis of the nucleotide sequence encoding the two external domains suggests that the membrane proximal domain has been subject to conservative selection, whereas replacement substitutions have been selected positively at certain residues within the amino terminal domain. The overall organization of the RT1.A beta gene is similar to that of the H-2A beta gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Genes H-2 Antigens/genetics HLA-DQ Antigens Histocompatibility Antigens Class II/genetics Major Histocompatibility Complex Protein Biosynthesis Rats/genetics Sequence Homology, Nucleic Acid
Chemicals
H-2 Antigens HLA-DQ Antigens Histocompatibility Antigens Class II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eccles S J
McMaster W R
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35 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00430314
Published
1985-00-00
Pages
653-63
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M15354, M15355, M15356, M15357, M15358
External Links
PubMed source DOI: 10.1007/BF00430314 View journal details
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