Enhanced thromboxane synthesis and vacuolization in human polymorphonuclear leucocytes induced by human lymphokine containing supernatants.

Clinical rheumatology ·Vol. 4 ·No. 4 ·1985-12-00 ·Pages 410-9

Conti P, Allegretta M, Williams TW, Cifone MG, Alesse E, Reale M, Boidi E, Dempsey RA

Abstract

PMN's were cultivated in vitro and treated with supernatants obtained from mitogenic-induced lymphocytes of human tonsil. Cytoplasmic vacuolization increased with time and there was a lower number of neutrophilic clumps in the culture treated with a supernatant containing lymphotoxin. In addition, the PMN's released more thromboxane B2 which was inhibited by indomethacin. We conclude that the action of lymphotoxin on the target PMN's is not mediated by the production of thromboxane B2.

MeSH Terms

Adult Animals Cattle Child Humans In Vitro Techniques Indomethacin/pharmacology Lymphocytes/drug effects Lymphokines/pharmacology Lymphotoxin-alpha/metabolism Mice Neutrophils/metabolism Organoids/metabolism Phytohemagglutinins/pharmacology Stimulation, Chemical Thromboxane B2/biosynthesis Time Factors Vacuoles/metabolism

Chemicals

Lymphokines Lymphotoxin-alpha Phytohemagglutinins Thromboxane B2 Indomethacin

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Conti P

Allegretta M

Williams T W

Cifone M G

Alesse E

Reale M

Boidi E

Dempsey R A

References (22)

22 references, click to expand

Partial reversal of the cellular immune defect in common variable immunodeficiency with indomethacin.
J Clin Lab Immunol. 1978 Nov;1(3):197-9 PMID: 756469
Target-cell membrane alterations induced by lymphotoxin. Ultrastructural observations.
Am J Pathol. 1977 Jan;86(1):149-62 PMID: 299796
Evolution and content of vacuoles in primary hypokalemic periodic paralysis.
Mayo Clin Proc. 1970 Nov-Dec;45(11):774-814 PMID: 4099018
Phospholipid, glycolipid, and ion dependencies of concanavalin A- and Ricinus communis agglutinin I-induced agglutination of lipid vesicles.
J Biol Chem. 1980 Jul 25;255(14):6766-71 PMID: 7391048
Aggregation of renal brush border membranes by concanavalin A and heavy metals.
Toxicol Appl Pharmacol. 1982 Jun 15;64(1):10-9 PMID: 7112572
Activation of membrane phospholipase A by guinea pig lymphotoxin (GLT).
J Immunol. 1979 Mar;122(3):791-4 PMID: 312824
Regulation of oxygen metabolism in human granulocytes: relationship between stimulus binding and oxidative response using plant lectins as probes.
Blood. 1980 Jun;55(6):1003-10 PMID: 7378576
Stimulation of polymorphonuclear leukocyte bactericidal activity by supernatants of activated human mononuclear cells.
Infect Immun. 1978 Nov;22(2):502-7 PMID: 730369
Isolation of lymphocytes, granulocytes and macrophages.
Scand J Immunol. 1976 Jun;Suppl 5:9-15 PMID: 1052391
Precursor role of arachidonic acid in release of slow reacting substance from rat basophilic leukemia cells.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4577-81 PMID: 22078
Lymphocyte-induced production of prostaglandin E2 by tumor cells in vitro: requirements for direct contact and lymphocyte viability.
Cell Immunol. 1980 Oct;55(2):428-35 PMID: 7428053
Early biochemical alterations induced by lymphotoxin in target cells.
J Immunol. 1973 Oct;111(4):1128-35 PMID: 4728679
Effect of concanavalin A on Ca2+ binding, uptake and the Ca2+ ATPase of lymphocyte plasma membranes.
Biochem Biophys Res Commun. 1980 Dec 16;97(3):1146-53 PMID: 6451223
In vitro lymphocyte cytotoxicity. I. Evidence of multiple cytotoxic molecules secreted by mitogen activated human lymphoid cells in vitro.
Cell Immunol. 1976 Jun 15;24(2):277-88 PMID: 1084788
Detection of lymphotoxins in vivo. I. Specific identification of short-lived LT activity in the plasma of various human patients employing rabbit anti-human LT sera in vitro.
Clin Immunol Immunopathol. 1978 May;10(1):104-15 PMID: 350462
Chemotactic responses of various differentiational stages of neutrophils from human cord and adult blood.
Infect Immun. 1982 Mar;35(3):921-8 PMID: 7068221
Isolation and characterization of an inhibitory glycopeptide against guinea pig lymphotoxin from the surface of L cells.
Immunochemistry. 1978 Jan;15(1):61-6 PMID: 304833
The human LT serum. X. The initial form released by T-enriched lymphocytes is 150,000 m.w., associated with small nonlytic components, and can dissociate into the smaller alpha, beta, and gamma m.w. classes.
J Immunol. 1981 Jun;126(6):2165-70 PMID: 6164723
Lymphocyte in vitro cytotoxicity: mechanism of human lymphotoxin-induced target cell destruction.
Cell Immunol. 1973 Feb;6(2):171-85 PMID: 4693834
The human LT system. II. Immunological relationships of LT molecules released by mitogen activated human lymphocytes in vitro.
Cell Immunol. 1978 Jul;38(2):403-16 PMID: 79447
Cytotoxic activity of lymphocytes. VI. Heterogeneity of cytotoxins in supernatants of mitogen-activated lymphocytes.
J Immunol. 1976 Mar;116(3):807-15 PMID: 1082895
Lymphocyte in vitro cytotoxicity: mechanisms of immune and non-immune small lymphocyte mediated target L cell destruction.
J Immunol. 1968 Jul;101(1):111-20 PMID: 5690881

Article Info

Journal

Clinical rheumatology

Abbr.

Clin Rheumatol

ISSN

0770-3198

DOI

10.1007/BF02031893

Published

1985-12-00

Pages

410-9

Language

English

Region

Germany

NLM ID

8211469

Subset

External Links

PubMed source DOI: 10.1007/BF02031893 View journal details

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Enhanced thromboxane synthesis and vacuolization in human polymorphonuclear leucocytes induced by human lymphokine containing supernatants.

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