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PMID: 388442 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Accessibility of guanine at position 44 in the invariant sequence 5'CCG44AAC3' of Escherichia coli 5S RNA to reaction with kethoxal.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4400-4

Larrinua I, Delihas N

Abstract

The reaction of Escherichia coli ribosomes with beta-ethoxy-alpha-ketobutyraldehyde (kethoxal) in a buffer containing 50--100 mM Tris.HCl at pH 7.4, 50 mM NH4Cl, and 5 mM Mg(OAc)2 readily released the 5S RNA from the ribosomes. When liberated, the 5S RNA is in a conformation such that position 44 is selectively reactive, in addition to the normally reactive quanines at positions 41 and 13. Positions 41 and 13 have been previously shown to react in the 5S RNA in situ. The resulting new RNase T1 resistant oligonucleotides 5'CCG 44K AAUCAG51(3') and 5'ACCCCAUG 41KCCG 44KAACUCAG51(3') have been isolated and identified. These oligonucleotides have not been found in RNase T1 digests of 5S RNA that is not released from the ribosome. The guanine at position 44 is part of the invariant sequence 5'CCG44AAC3' which includes that portion of the molecule thought to interact with the invariant 5'GT psi C3' of tRNAs in the ribosomal A site. This invariant sequence of the 5S RNA may also form part of the binding site for protein L5.

MeSH Terms
Aldehydes/pharmacology Base Sequence Butanones/pharmacology Chemical Phenomena Chemistry Escherichia coli/ultrastructure Guanine Nucleotides RNA, Ribosomal/metabolism Ribosomal Proteins/metabolism Ribosomes/drug effects,metabolism,ultrastructure
Chemicals
Aldehydes Butanones Guanine Nucleotides RNA, Ribosomal Ribosomal Proteins kethoxal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Larrinua I
Delihas N
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4400
Published
1979-09-00
Pages
4400-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411582
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4400 PMC Full Text View journal details
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