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PMID: 3884513 Published · ppublish English Comparative Study Journal Article

Nucleotide sequence comparison between heat-labile toxin B-subunit cistrons from Escherichia coli of human and porcine origin.

Infection and immunity ·Vol. 48 ·No. 1 ·1985-04-00 ·Pages 73-7

Leong J, Vinal AC, Dallas WS

Abstract

The nucleotide sequence of the LT-BH cistron (eltBH) from an enterotoxigenic Escherichia coli strain infectious for humans was determined and compared with the LT-B cistron sequence from a porcine E. coli isolate. Both cistrons were shown to comprise 375 nucleotide base pairs, and discrepancies were detected at eight positions. Of the nonhomologous base pairs, six resulted in codon changes that would lead to amino acid variations. The nucleotide sequence distal to both LT-B cistrons was also determined, and only three differences were detected in 197 base pairs. An HhaI site unique to eltBH was shown to be present in all the heat-labile (LT) genes from 31 human isolates surveyed, whereas the restriction enzyme recognition site was absent in the gene from 46 porcine E. coli isolates. The results suggest that two genetically discernable LT groups are identifiable and that the groups are also distinguishable by the isolation source (human or porcine) of the infecting E. coli strains.

MeSH Terms
Amino Acid Sequence Animals Bacterial Toxins/genetics Base Sequence DNA, Bacterial/analysis Enterotoxins/genetics Escherichia coli/genetics Escherichia coli Proteins Genes Humans Polymorphism, Genetic Species Specificity Swine/microbiology
Chemicals
Bacterial Toxins DNA, Bacterial Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leong J
Vinal A C
Dallas W S
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37 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.48.1.73-77.1985
Published
1985-04-00
Pages
73-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261916
Subset
IM
Databases
GENBANK
J01605, M17873, M17874
External Links
PubMed source DOI: 10.1128/iai.48.1.73-77.1985 PMC Full Text View journal details
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