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PMID: 3894329 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in the spoT gene of Salmonella typhimurium: effects on his operon expression.

Journal of bacteriology ·Vol. 163 ·No. 2 ·1985-08-00 ·Pages 534-42

Rudd KE, Bochner BR, Cashel M, Roth JR

Abstract

The spoT gene of Salmonella typhimurium has been identified. Mutations in spoT map between gltC and pyrE at 79 min. The spoT1 mutant has elevated levels of guanosine 5'-diphosphate-3'-diphosphate (ppGpp) during steady-state growth and exhibits a slower than normal decay of ppGpp after reversal of amino acid starvation. The spoT1 mutation elevates his operon expression but is distinct from known his regulatory mutations. Elevated his operon expression in spoT mutants causes resistance to the histidine analogs, 1,2,4-triazole-3-alanine and 3-amino-1,2,4-triazole. These properties of spoT mutants allowed us to identify and characterize additional spoT mutants. Approximately 40% of these mutants are temperature sensitive for growth on minimal medium, suggesting that the spoT function is essential or that excessive accumulation of ppGpp is lethal.

MeSH Terms
Alcohol Oxidoreductases/genetics Alleles Genes Genes, Bacterial Genes, Regulator Genotype Guanosine Tetraphosphate/metabolism Kinetics Mutation Operon Salmonella typhimurium/genetics,metabolism Species Specificity Temperature
Chemicals
Guanosine Tetraphosphate Alcohol Oxidoreductases histidinol dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rudd K E
Bochner B R
Cashel M
Roth J R
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.2.534-542.1985
Published
1985-08-00
Pages
534-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219155
Subset
IM
Grants
NIGMS NIH HHS · GM19993 · United States
NIGMS NIH HHS · GM23408 · United States
External Links
PubMed source DOI: 10.1128/jb.163.2.534-542.1985 PMC Full Text View journal details
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