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PMID: 3895228 Published · ppublish English Journal Article

Antitermination by both the promoter and the leader regions of an Escherichia coli ribosomal RNA operon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 15 ·1985-08-00 ·Pages 5073-7

Holben WE, Prasad SM, Morgan EA

Abstract

RNA polymerase initiating at Escherichia coli ribosomal RNA promoter-leader regions can efficiently read through factor rho-dependent termination signals. Dissection of the promoter-leader region reveals that the ability to read through termination signals is conferred independently by both promoter and leader regions. Events in the leader also affect the transcription rate of structural genes downstream of the leader. When cells are grown in rich medium, the rrnC leader reduces transcription by a factor of approximately 4 when downstream of the rrnC promoters and by a factor of 2 when downstream of the lac promoter.

MeSH Terms
Base Sequence DNA, Recombinant DNA, Ribosomal/genetics Escherichia coli/genetics Operon Plasmids Promoter Regions, Genetic RNA, Ribosomal/genetics Rho Factor/genetics Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Ribosomal RNA, Ribosomal Rho Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Holben W E
Prasad S M
Morgan E A
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.15.5073
Published
1985-08-00
Pages
5073-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390501
Subset
IM
Databases
GENBANK
M10739
External Links
PubMed source DOI: 10.1073/pnas.82.15.5073 PMC Full Text View journal details
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