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PMID: 6091131 Published · ppublish English Journal Article

In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 6009-13

Peacock S, Weissbach H, Nash HA

Abstract

The effect of Escherichia coli integration host factor (IHF) on phage lambda gene expression has been examined in a simplified DNA-directed in vitro system that measures the formation of the first dipeptide of the gene product. Plasmid pKC30cII, which contains the phage lambda genes N, cII and O, under control of the PL promoter, was used as template to study the expression of the first dipeptide of the gene products--i.e., fMet-Asp for N protein, fMet-Val for cII, and fMet-Thr for O. Purified IHF stimulates the DNA-directed synthesis of fMet-Val (cII) and fMet-Thr (O) 2-3-fold but has no effect on the synthesis of fMet-Asp (N). In this in vitro system, the stimulation by IHF of cII and O gene expression is at the level of transcription. Phage lambda repressor completely inhibits dipeptide synthesis in the presence or absence of IHF. The results are consistent with a role of IHF as a transcription antiterminator, perhaps functioning at or near the tR1 site preceding the cII gene.

MeSH Terms
Bacteriophage lambda/genetics DNA Transposable Elements DNA-Directed RNA Polymerases/metabolism Dipeptides/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Viral Integrases Kinetics Plasmids Protein Biosynthesis RNA, Messenger/genetics Transcription Factors/genetics Viral Proteins/metabolism
Chemicals
DNA Transposable Elements Dipeptides RNA, Messenger Transcription Factors Viral Proteins cII protein, bacteriophage lambda Integrases DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peacock S
Weissbach H
Nash H A
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.6009
Published
1984-10-00
Pages
6009-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391848
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.19.6009 PMC Full Text View journal details
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