Home LiteratureArticle Details

PMID: 3904742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Membrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Purification and properties.

The Biochemical journal ·Vol. 231 ·No. 2 ·1985-10-15 ·Pages 407-16

Allison N, O'Donnell MJ, Fewson CA

Abstract

Procedures were developed for the optimal solubilization of D-lactate dehydrogenase, D-mandelate dehydrogenase, L-lactate dehydrogenase and L-mandelate dehydrogenase from wall + membrane fractions of Acinetobacter calcoaceticus. D-Lactate dehydrogenase and D-mandelate dehydrogenase were co-eluted on gel filtration, as were L-lactate dehydrogenase and L-mandelate dehydrogenase. All four enzymes could be separated by ion-exchange chromatography. D-Lactate dehydrogenase and D-mandelate dehydrogenase were purified by cholate extraction, (NH4)2SO4 fractionation, gel filtration, ion-exchange chromatography and chromatofocusing. The properties of D-lactate dehydrogenase and D-mandelate dehydrogenase were similar in several respects: they had relative molecular masses of 62 800 and 59 700 respectively, pI values of 5.8 and 5.5, considerable sensitivity to p-chloromercuribenzoate, little or no inhibition by chelating agents, and similar responses to pH. Both enzymes appeared to contain non-covalently bound FAD as cofactor.

MeSH Terms

Acinetobacter/enzymology Alcohol Oxidoreductases/antagonists & inhibitors,isolation & purification,metabolism Cell Membrane/enzymology Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Flavins/metabolism Isoenzymes/isolation & purification,metabolism L-Lactate Dehydrogenase/antagonists & inhibitors,isolation & purification,metabolism Substrate Specificity

Chemicals

Flavins Isoenzymes Alcohol Oxidoreductases D-mandelate dehydrogenase L-Lactate Dehydrogenase L-mandelate dehydrogenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Allison N

O'Donnell M J

Fewson C A

References (23)

23 references, click to expand

The growth and metabolic versatility of the gram-negative Bacterium NCIB 8250 ("Vibrio 01").
J Gen Microbiol. 1967 Feb;46(2):255-66 PMID: 6029734
THE MOLAR EXTINCTION COEFFICIENT OF 2,6-DICHLOROPHENOL INDOPHENOL.
Biochim Biophys Acta. 1964 Apr 4;86:194-7 PMID: 14166862
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties.
Biochemistry. 1973 Jun 19;12(13):2468-74 PMID: 4575624
Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli.
J Biol Chem. 1973 Oct 25;248(20):7012-7 PMID: 4582730
Solubilization of membranes by detergents.
Biochim Biophys Acta. 1975 Mar 25;415(1):29-79 PMID: 1091302
D-Lactate dehydrogenase of Peptostreptococcus elsdenii.
J Bacteriol. 1975 Dec;124(3):1454-61 PMID: 368
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Characterization of membrane proteins in detergent solutions.
Biochim Biophys Acta. 1976 Oct 26;457(2):133-70 PMID: 135582
The subunit structure of the arom multienzyme complex of Neurospora crassa. A possible pentafunctional polypeptide chain.
Biochem J. 1977 Mar 1;161(3):599-607 PMID: 139889
Inducible membrane-bound L-lactate dehydrogenase from Escherichia coli. Purification and properties.
J Biol Chem. 1977 Aug 25;252(16):5820-7 PMID: 18473
Membrane-bound D-lactate dehydrogenase from Escherichia coli: purification and properties.
Biochemistry. 1979 Jan 23;18(2):312-6 PMID: 369599
Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii.
Biochemistry. 1979 Oct 16;18(21):4714-24 PMID: 497162
Phenazine ethosulfate as a preferred electron acceptor to phenazine methosulfate in dye-linked enzyme assays.
Anal Biochem. 1979 Oct 15;99(1):112-7 PMID: 231392
Bacterial lactate dehydrogenases.
Microbiol Rev. 1980 Mar;44(1):106-39 PMID: 6997721
Effects of solubilisation on some properties of the membrane-bound respiratory enzyme D-amino acid dehydrogenase of Escherichia coli.
FEBS Lett. 1983 Jan 24;151(2):189-92 PMID: 6131836
Mutant strains of Acinetobacter calcoaceticus possessing additional mandelate dehydrogenases. Identification and preliminary characterization of the enzymes.
Biochem J. 1983 Feb 1;209(2):379-86 PMID: 6342613
Regulation of expression of novel mandelate dehydrogenases in mutants of Acinetobacter calcoaceticus.
J Gen Microbiol. 1983 Jul;129(7):2009-15 PMID: 6355386
Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli.
Eur J Biochem. 1984 Oct 15;144(2):367-73 PMID: 6386470
Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.
J Bacteriol. 1985 Mar;161(3):1059-68 PMID: 3882663
Membrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Location and regulation of expression.
Biochem J. 1985 May 1;227(3):753-7 PMID: 3890833
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2310407

Published

1985-10-15

Pages

407-16

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152761

Subset

External Links

PubMed source DOI: 10.1042/bj2310407 PMC Full Text View journal details

Analysis Services

Membrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Purification and properties.

Contact

WeChat Official Account