Home LiteratureArticle Details
PMID: 3906568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the trfA and trfB promoters of broad host range plasmid RK2: identification of sequences essential for regulation by trfB/korA/korD.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8129-42

Theophilus BD, Cross MA, Smith CA, Thomas CM

Abstract

Using a plasmid containing a transcriptional fusion in which the E.coli galK gene is expressed from the trfB promoter of broad host range plasmid RK2 we show that transcription from the trfB promoter is repressed by the products of both the trfB and korB genes as we have previously predicted from the sequence homology of the trfA and trfB promoters and the fact that the trfA promoter is regulated by trfB and korB. These loci, trfB and korB are normally transcribed from the trfB promoter. Thus the trfB incC korB operon of RK2 is doubly autogeneously regulated. In addition, we describe the isolation and characterization of a mutant trfA promoter which has become insensitive to repression by trfB as a result of a point mutation within the inverted repeat sequence previously predicted to be the trfB protein binding site. These results provide strong evidence for our previously proposed model for control of transcription from the trfA and trfB promoters.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Base Sequence Escherichia coli/genetics Galactokinase/biosynthesis,genetics Genes, Bacterial Operon Promoter Regions, Genetic R Factors Repressor Proteins/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Repressor Proteins Galactokinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Theophilus B D
Cross M A
Smith C A
Thomas C M
References (35)
35 references, click to expand
  1. Molecular genetics of broad host range plasmid RK2.
    Plasmid. 1981 Jan;5(1):10-9 PMID: 6164066
  2. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.
    Gene Amplif Anal. 1981;2:383-415 PMID: 6101056
  3. Complementation analysis of replication and maintenance functions of broad host range plasmids RK2 and RP1.
    Plasmid. 1981 May;5(3):277-91 PMID: 7022504
  4. Plasmid RP4 specifies a deoxyribonucleic acid primase involved in its conjugal transfer and maintenance.
    J Bacteriol. 1981 Dec;148(3):769-81 PMID: 6273381
  5. Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1935-9 PMID: 7043468
  6. Maintenance of broad host range plasmid RK2 replicons in Pseudomonas aeruginosa.
    Nature. 1982 Aug 12;298(5875):674-6 PMID: 6285208
  7. Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2.
    J Bacteriol. 1982 Dec;152(3):1078-90 PMID: 6754696
  8. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  9. Deletion mapping of kil and kor functions in the trfA and trfB regions of broad host range plasmid RK2.
    Mol Gen Genet. 1983;190(2):245-54 PMID: 6308390
  10. Essential genes of plasmid RK2 in Escherichia coli: trfB region controls a kil gene near trfA.
    J Bacteriol. 1983 Nov;156(2):584-91 PMID: 6313609
  11. Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous plasmid RK2.
    Nucleic Acids Res. 1983 Nov 11;11(21):7453-69 PMID: 6316262
  12. Gene regulation in plasmid RK2: positive control by korA in the expression of korC.
    J Bacteriol. 1984 Jan;157(1):247-52 PMID: 6360998
  13. Molecular cloning and mapping of SphI restriction fragments of plasmid RP4.
    Plasmid. 1983 Nov;10(3):303-7 PMID: 6318250
  14. Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.
    EMBO J. 1982;1(11):1399-404 PMID: 6327267
  15. Unidirectional replication of the P-group plasmid RK2.
    Biochim Biophys Acta. 1977 Sep 6;478(1):109-13 PMID: 329885
  16. Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion.
    J Bacteriol. 1978 Jan;133(1):43-52 PMID: 338595
  17. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K.
    Cell. 1978 Dec;15(4):1199-208 PMID: 728998
  18. Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification.
    Mol Gen Genet. 1979 Apr 17;172(1):53-65 PMID: 377024
  19. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical.
    J Gen Microbiol. 1979 Oct;114(2):341-8 PMID: 120408
  22. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.
    Methods Enzymol. 1979;68:268-80 PMID: 232215
  23. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  24. Replication and incompatibility properties of segments of the origin region of replication of the broad host range plasmid RK2.
    Mol Gen Genet. 1981;181(1):1-7 PMID: 6261080
  25. Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2.
    Mol Gen Genet. 1981;181(1):8-12 PMID: 6261086
  26. The DNA-protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer.
    Mol Gen Genet. 1979 Oct 3;176(2):183-9 PMID: 393953
  27. Analysis of the trfA region of broad host-range plasmid RK2 by transposon mutagenesis and identification of polypeptide products.
    J Mol Biol. 1984 May 25;175(3):229-49 PMID: 6327994
  28. Nucleotide sequence of the trfA gene of broad host-range plasmid RK2.
    J Mol Biol. 1984 May 25;175(3):251-62 PMID: 6374158
  29. The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences.
    Nucleic Acids Res. 1984 Apr 25;12(8):3619-30 PMID: 6328430
  30. The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression.
    EMBO J. 1984 Jul;3(7):1513-9 PMID: 6378626
  31. Molecular gentic analysis of the trfB and korB region of broad host range plasmid RK2.
    J Gen Microbiol. 1984 Jul;130(7):1651-63 PMID: 6381647
  32. Transcription in the trfA region of broad host range plasmid RK2 is regulated by trfB and korB.
    Mol Gen Genet. 1984;195(3):523-9 PMID: 6088948
  33. Replication control in promiscuous plasmid RK2: kil and kor functions affect expression of the essential replication gene trfA.
    J Bacteriol. 1985 Jul;163(1):228-37 PMID: 3891728
  34. Genetic interactions of broad host-range plasmid RK2: evidence for a complex replication regulon.
    Basic Life Sci. 1985;30:227-41 PMID: 2990411
  35. Complementation analysis in Pseudomonas aeruginosa of the transfer genes of the wide host range R plasmid R18.
    Plasmid. 1981 Mar;5(2):202-12 PMID: 6264523
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8129
Published
1985-11-25
Pages
8129-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322115
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.22.8129 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2