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PMID: 3906569 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence of the Saccharomyces cerevisiae PHR1 gene and homology of the PHR1 photolyase to E. coli photolyase.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8231-46

Sancar GB

Abstract

The nucleotide sequence of a 2301 base pair region of Saccharomyces cerevisiae DNA containing the PHR1 gene is reported. Within this region a single open reading frame of 1695 base pairs was found; using the insertional inactivation technique it was shown that part or all of this open reading frame specifies the PHR1-encoded photolyase. The amino acid sequence of the 565 amino acid long polypeptide predicted from the PHR1 nucleotide sequence was compared to the amino acid sequence of E. coli photolyase. Overall the sequence homology was 36.5%; however, two short regions near the amino terminus as well as the carboxy-terminal 150 amino acids display significantly greater sequence homology. The presence of these strongly conserved regions suggests that the yeast and E. coli photolyase possess common structural and functional domains involved in substrate and/or chromophore binding.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Binding Sites Codon Deoxyribodipyrimidine Photo-Lyase/genetics Escherichia coli/enzymology,genetics Fungal Proteins/genetics Genes, Fungal Lyases/genetics Recombinant Proteins/genetics Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Codon Fungal Proteins Recombinant Proteins Lyases Deoxyribodipyrimidine Photo-Lyase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Sancar G B
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8231
Published
1985-11-25
Pages
8231-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322122
Subset
IM
Grants
NIGMS NIH HHS · GM31082 · United States
NIGMS NIH HHS · GM35123 · United States
Databases
GENBANK
M20914, X03183
External Links
PubMed source DOI: 10.1093/nar/13.22.8231 PMC Full Text View journal details
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