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PMID: 2987851 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae: a potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region.

Nucleic acids research ·Vol. 13 ·No. 7 ·1985-04-11 ·Pages 2357-72

Reynolds P, Higgins DR, Prakash L, Prakash S

Abstract

The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5' end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89,779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair.

MeSH Terms
Adenine Nucleotides/metabolism Amino Acid Sequence Base Sequence DNA/metabolism DNA Restriction Enzymes/metabolism Plasmids RNA, Messenger/analysis Saccharomyces cerevisiae/genetics,radiation effects Ultraviolet Rays
Chemicals
Adenine Nucleotides RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reynolds P
Higgins D R
Prakash L
Prakash S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.7.2357
Published
1985-04-11
Pages
2357-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341160
Subset
IM
Grants
NCI NIH HHS · CA35035 · United States
NIGMS NIH HHS · GM19261 · United States
Databases
GENBANK
X02368
External Links
PubMed source DOI: 10.1093/nar/13.7.2357 PMC Full Text View journal details
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