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PMID: 6759871 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 188 ·No. 2 ·1982-00-00 ·Pages 235-9

Miller RD, Prakash L, Prakash S

Abstract

Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-delta 1, rad23-delta 1, and rad7-delta 1 rad23-delta 1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

MeSH Terms
Biological Evolution DNA Repair DNA, Fungal/radiation effects Furocoumarins Genes Mutation Pyrimidine Dimers/metabolism Saccharomyces cerevisiae/genetics Ultraviolet Rays
Chemicals
DNA, Fungal Furocoumarins Pyrimidine Dimers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller R D
Prakash L
Prakash S
References (18)
18 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332681
Published
1982-00-00
Pages
235-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · 1RO1 CA32514 · United States
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source DOI: 10.1007/BF00332681
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