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PMID: 6752694 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic control of excision of Saccharomyces cerevisiae interstrand DNA cross-links induced by psoralen plus near-UV light.

Molecular and cellular biology ·Vol. 2 ·No. 8 ·1982-08-00 ·Pages 939-48

Miller RD, Prakash L, Prakash S

Abstract

Excision of interstrand DNA cross-links induced by 4,5',8-trimethyl psoralen plus 360-nm light was examined in wild type (RAD+) and various radiation-sensitive (rad) mutants of Saccharomyces cerevisiae known to be defective in the excision of UV light-induced pyrimidine dimers. Alkaline sucrose sedimentation of DNA after incubation of psoralen-plus-light-treated cells indicated little or no nicking of cross-linked DNA in rad1-2, rad2-5, rad3-2, rad4-4, rad10-2, and mms19-1 mutants. In the rad14-2 mutant, substantial nicking was observed but to a much lesser extent than in the RAD+ strains, whereas the rad16-1 mutant was as proficient in nicking as the RAD+ strain. Removal of cross-links was also examined in RAD+, rad3-2, and rad14-2 strains by determining the sensitivity of alkali-denatured and -neutralized DNA to hydrolysis by S1 nuclease. No cross-link removal was observed in the rad3-2 mutants, and the rad14-2 mutant was much less efficient than the RAD+ strain in removing cross-links.

MeSH Terms
DNA Repair DNA, Fungal/metabolism Furocoumarins/pharmacology Models, Biological Mutation Pyrimidine Dimers/metabolism Saccharomyces cerevisiae/drug effects,genetics,radiation effects Trioxsalen/pharmacology Ultraviolet Rays
Chemicals
DNA, Fungal Furocoumarins Pyrimidine Dimers Trioxsalen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller R D
Prakash L
Prakash S
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53 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.8.939-948.1982
Published
1982-08-00
Pages
939-48
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369882
Subset
IM
Grants
NCI NIH HHS · 1RO1 CA32514 · United States
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.8.939-948.1982 PMC Full Text View journal details
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