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PMID: 7017347 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard.

Molecular & general genetics : MGG ·Vol. 181 ·No. 3 ·1981-00-00 ·Pages 346-51

Ruhland A, Haase E, Siede W, Brendel M

Abstract

Mutants of Saccharomyces cerevisiae with enhanced sensitivity to the DNA cross-linking agent nitrogen mustard (HN2) have been isolated and partially characterized with respect to their phenotypic and genetic properties. The screening technique, based on HN2-sensitivity as sole criterion, yields approxiamtely 1 sensitive isolate in 200 clones when applied to an intensively mutagenized population of a resistant parent strain. Mutants characterized so far are all due to recessive nuclear genes and represent at least seven complementation groups. They exhibit different degrees as well as different patterns of sensitivity towards monofunctional and bifunctional alkylating agents, and ultraviolet light.

MeSH Terms

Alkylating Agents/toxicity Genetic Linkage Mutation Nitrogen Mustard Compounds/pharmacology Phenotype Saccharomyces cerevisiae/drug effects,genetics

Chemicals

Alkylating Agents Nitrogen Mustard Compounds

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ruhland A

Haase E

Siede W

Brendel M

References (14)

14 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425609

Published

1981-00-00

Pages

346-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00425609

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Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard.

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