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PMID: 3915784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of a cell surface immobilization antigen during serotype transformation in Tetrahymena thermophila.

Molecular and cellular biology ·Vol. 5 ·No. 8 ·1985-08-00 ·Pages 1925-32

Williams NE, Doerder FP, Ron A

Abstract

A temperature shift from 40 to 28 degrees C rapidly induced expression of a specific immobilization antigen at the cell surface in Tetrahymena thermophila. This transformation was inhibited by actinomycin D and cycloheximide but not by colchicine or cytochalasin B. The major surface antigen expressed at 28 degrees C in cells homozygous for the SerH3 allele was partially purified, and an antiserum against this preparation was raised in rabbits. Electrophoresis, immunoblot, and [35S]methionine incorporation studies are reported which support the conclusion that the H3 antigen is an acidic protein with an Mr of approximately 52,000 daltons. An induced synthesis of the H3 immobilization antigen was detected within 30 min after a shift from 40 to 28 degrees C. This protein appeared to be synthesized in the microsomal fraction and transferred without cleavage to the cell surface, where it was inserted first into nonciliated regions.

MeSH Terms
Animals Antigens, Protozoan Antigens, Surface/analysis Cell Membrane/immunology,ultrastructure Colchicine/pharmacology Cytochalasin B/pharmacology Immune Sera Immunologic Techniques Microscopy, Electron Protozoan Proteins Temperature Tetrahymena/drug effects,immunology,ultrastructure
Chemicals
Antigens, Protozoan Antigens, Surface Immune Sera Protozoan Proteins immobilization antigens Cytochalasin B Colchicine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams N E
Doerder F P
Ron A
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21 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.8.1925-1932.1985
Published
1985-08-00
Pages
1925-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366909
Subset
IM
Grants
NIGMS NIH HHS · GM-29588 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.8.1925-1932.1985 PMC Full Text View journal details
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