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PMID: 4933471 Published · ppublish English Journal Article

Studies of membrane formation in Tetrahymena pyriformis. II. Isolation and lipid analysis of cell fractions.

The Journal of cell biology ·Vol. 49 ·No. 3 ·1971-06-00 ·Pages 712-21

Nozawa Y, Thompson GA

Abstract

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.

MeSH Terms
Animals Cell Membrane/analysis Chromatography, Thin Layer Cilia/analysis Culture Media Histocytochemistry Histological Techniques Lipids/analysis Methods Microscopy, Electron Microsomes/analysis Mitochondria/analysis Phospholipids/analysis Tetrahymena/cytology
Chemicals
Culture Media Lipids Phospholipids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nozawa Y
Thompson G A
References (21)
21 references, click to expand
  1. Fine structure, reconstruction and possible functions of components of the cortex of Tetrahymena pyriformis.
    J Protozool. 1967 Nov;14(4):553-65 PMID: 5604474
  2. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  3. Heterogeneity of rough-surfaced liver microsomal membranes of adult, phenobarbital-treated, and newborn rats.
    J Cell Biol. 1968 Aug;38(2):257-76 PMID: 4385751
  4. Ribonucleic acid synthesis in isolated mitochondria from Tetrahymena.
    J Biol Chem. 1968 Jan 25;243(2):320-8 PMID: 4966233
  5. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  6. Respiratory enzyme studies in Tetrahymena pyriformis. 4. Stabilization of electron-transport components.
    Biochem J. 1959 Jan;71(1):106-18 PMID: 13628540
  7. The chromatographic separation of phosphonolipids from their phospholipid analogs.
    Biochim Biophys Acta. 1969 Mar 4;176(2):324-9 PMID: 4304772
  8. The properties of an enzyme system degrading endogenous phospholipids of Tetrahymena pyriformis.
    J Protozool. 1969 Aug;16(3):397-400 PMID: 5343456
  9. Studies of membrane formation in Tetrahymena pyriformis. II. Isolation and lipid analysis of cell fractions.
    J Cell Biol. 1971 Jun;49(3):712-21 PMID: 4933471
  10. Studies of membrane formation in Tetrahymena pyriformis. I. Rates of phospholipid biosynthesis.
    Biochemistry. 1967 Jul;6(7):2015-22 PMID: 6049442
  11. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  12. Phosphonolipids in Tetrahymena cilia.
    Biochem Biophys Res Commun. 1970;39(6):1163-9 PMID: 5513252
  13. Serum lipide analysis by chromatography and infrared spectrophotometry.
    J Biol Chem. 1957 Jul;227(1):449-64 PMID: 13449086
  14. Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots.
    Lipids. 1966 Jan;1(1):85-6 PMID: 17805690
  15. Phosphonolipids: localization in surface membranes of Tetrahymena.
    Science. 1970 May 22;168(3934):989-91 PMID: 5441031
  16. Fractionation of nuclei from Paramecium aurelia by density gradient centrifugation.
    Biochim Biophys Acta. 1969 Nov 19;195(1):268-70 PMID: 5357035
  17. Membranes of animal cells. IV. Lipids of the L cell and its surface membrane.
    J Biol Chem. 1969 Aug 10;244(15):4103-11 PMID: 4308167
  18. A method for quantitative determination of phosphonate phosphorus in the presence of organic anc inorganic phosphates.
    Anal Biochem. 1968 Sep;24(3):443-7 PMID: 4301971
  19. Preparation and properties of mitochondria from the ciliated protozoan, Tetrahymena.
    J Biochem. 1965 Nov;58(5):444-57 PMID: 5866271
  20. Lipid composition of mitochondria from bovine heart, liver, and kidney.
    J Lipid Res. 1967 May;8(3):170-80 PMID: 4292227
  21. The metabolism of 2-aminoethylphosphonate lipids in Tetrahymena pyriformis.
    Biochim Biophys Acta. 1969 Mar 4;176(2):330-8 PMID: 5775950
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.49.3.712
Published
1971-06-00
Pages
712-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108490
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.49.3.712 PMC Full Text View journal details
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