Immunity and protection of mice against Neisseria meningitidis group B by vaccination, using polysaccharide complexed with outer membrane proteins: a comparison with purified B polysaccharide.

Infection and immunity ·Vol. 47 ·No. 2 ·1985-02-00 ·Pages 527-33

Moreno C, Lifely MR, Esdaile J

Abstract

A methodology for preparing outer membrane proteins (type specific) complexed to group B polysaccharide of Neisseria meningitidis is described. These complexes, low in nucleic acid and lipopolysaccharide content, were immunogenic in mice with induction of humoral antigroup B and antiprotein responses. Immunized mice were also protected against challenge with N. meningitidis group B strains of the same or a different type from that used for vaccination. Both immunity and protection were enhanced when the mice received a secondary immunization with the protein-polysaccharide complex. Additional data have shown the capacity of purified B polysaccharide to induce immunological memory, even though it is incapable of inducing a humoral response when given alone.

MeSH Terms

Animals Antibodies, Bacterial/analysis Antibody Formation Bacterial Capsules Bacterial Outer Membrane Proteins/administration & dosage,immunology Bacterial Vaccines/immunology,isolation & purification Female Immunization Immunologic Memory Meningococcal Infections/prevention & control Mice Neisseria meningitidis/immunology Polysaccharides, Bacterial/administration & dosage,immunology

Chemicals

Antibodies, Bacterial Bacterial Outer Membrane Proteins Bacterial Vaccines Polysaccharides, Bacterial capsular polysaccharide, meningococcal group B

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Moreno C

Lifely M R

Esdaile J

References (31)

31 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.47.2.527-533.1985

Published

1985-02-00

Pages

527-33

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC263203

Subset

External Links

PubMed source DOI: 10.1128/iai.47.2.527-533.1985 PMC Full Text View journal details

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Immunity and protection of mice against Neisseria meningitidis group B by vaccination, using polysaccharide complexed with outer membrane proteins: a comparison with purified B polysaccharide.

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