Identification of a gene, tir of R100, functionally homologous to the F3 gene of F in the inhibition of RP4 transfer.

Molecular & general genetics : MGG ·Vol. 198 ·No. 2 ·1985-00-00 ·Pages 356-7

Tanimoto K, Iino T, Ohtsubo H, Ohtsubo E

Abstract

We detected a gene of R100 functionally homologous to the F3 gene of F in the inhibition of RP4 transfer. Using in vitro recombinant DNA techniques, we located the gene, designated tir, in a 0.9 kb region, 2,392-3,293 in the nucleotide sequence coordinate of R100. From the DNA sequence analysis of R100 (Ohtsubo unpublished results), a coding frame of polypeptides, whose molecular weight is estimated to be 24.1 kilodaltons (kd), was inferred to be the region tir. Furthermore, we showed that tir could not repress expression of the F3 gene.

MeSH Terms

Conjugation, Genetic Escherichia coli/genetics F Factor Gene Expression Regulation Genes, Bacterial beta-Galactosidase/genetics

Chemicals

beta-Galactosidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tanimoto K

Iino T

Ohtsubo H

Ohtsubo E

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00383019

Published

1985-00-00

Pages

356-7

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00383019

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Identification of a gene, tir of R100, functionally homologous to the F3 gene of F in the inhibition of RP4 transfer.

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