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PMID: 3929254 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin 2 promotes growth and cytolytic activity in human T3+4-8- thymocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6268-71

de la Hera A, Toribio ML, Marquez C, Martinez C

Abstract

Human thymocytes bearing T3 but neither T4 nor T8 antigens (T3+4-8- cells) were obtained after negative selection of thymocytes, either fresh or cultured in medium containing recombinant interleukin 2 (IL-2), by treatment with Na1/34, OKT4A and B9.4 monoclonal antibodies (which recognize T6, T4, and T8 antigens, respectively) and complement. Quantitative flow cytometry showed a 98% pure population of T3+4-8- lymphocytes, which included proliferating cells. The growth and maturation requirements of these thymocytes were characterized and related to the T3-receptor complex and IL-2 pathways, thought to be used by mature lymphocytes. The results show that addition of recombinant IL-2 promotes, in a dose-dependent way, proliferation and acquisition of effector functions by cultured T3+4-8- thymocytes, the growth being inhibitable by monoclonal antibody 33B73 (anti-Tac). Furthermore, cytolytic activity of T3+4-8- cells induced by recombinant IL-2 is specifically blocked by monoclonal antibody OKT3, showing that it operates via the T3-receptor complex and does not require either T4 or T8 molecules. The finding of in vitro responsiveness to recombinant IL-2 in T3+4-8- thymocytes suggests a role of IL-2 in the growth and maturation of cells committed to the T-cell lineage, during intrathymic differentiation, prior to expression of T4 and T8 molecules.

MeSH Terms
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface/immunology Cell Differentiation Cell Division Flow Cytometry Humans Interleukin-2/physiology Receptors, Antigen, T-Cell/immunology Receptors, Immunologic/immunology Receptors, Interleukin-2 T-Lymphocytes, Cytotoxic/cytology,immunology Thymus Gland/cytology,immunology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface Interleukin-2 Receptors, Antigen, T-Cell Receptors, Immunologic Receptors, Interleukin-2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de la Hera A
Toribio M L
Marquez C
Martinez C
References (32)
32 references, click to expand
  1. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 1978 May;14(1):9-20 PMID: 667938
  2. Cell surface antigen marking the stages of murine T cell ontogeny and its functional subsets.
    Immunol Rev. 1984 Dec;82:117-39 PMID: 6241595
  3. The differentiation and function of human T lymphocytes.
    Cell. 1980 Apr;19(4):821-7 PMID: 6991122
  4. A suggested mechanism for T lymphocyte activation: implications on the acquisition of functional reactivities.
    Immunol Rev. 1980;51:61-91 PMID: 6159302
  5. Does OKT3 monoclonal antibody react with an antigen-recognition structure on human T cells?
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1805-8 PMID: 6165018
  6. Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.
    J Exp Med. 1981 Mar 1;153(3):595-604 PMID: 6788878
  7. Inhibition of specific cell-mediated cytotoxicity by monoclonal antibodies to human T cell antigens.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4500-4 PMID: 6974866
  8. Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies.
    J Exp Med. 1982 May 1;155(5):1579-84 PMID: 6978378
  9. Surface structures involved in target recognition by human cytotoxic T lymphocytes.
    Science. 1982 Oct 29;218(4571):471-3 PMID: 6981845
  10. Human cytotoxic T cell structures associated with expression of cytolysis. I. Analysis at the clonal cell level of the cytolysis-inhibiting effect of 7 monoclonal antibodies.
    Eur J Immunol. 1982 Sep;12(9):739-47 PMID: 6982820
  11. Antigen-specific human T-cell hybridomas with helper activity.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6646-50 PMID: 6983073
  12. Possible involvement of the OKT4 molecule in T cell recognition of class II HLA antigens. Evidence from studies of cytotoxic T lymphocytes specific for SB antigens.
    J Exp Med. 1982 Oct 1;156(4):1065-76 PMID: 6984061
  13. Xenogeneic human anti-mouse T cell responses are due to the activity of the same functional T cell subsets responsible for allospecific and major histocompatibility complex-restricted responses.
    J Exp Med. 1983 Feb 1;157(2):720-9 PMID: 6185618
  14. Differentiation in vitro of Lyt 2+ thymocytes from embryonic Lyt 2- precursors.
    Nature. 1983 May 19-25;303(5914):248-50 PMID: 6601775
  15. Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor.
    Nature. 1983 Jun 30;303(5920):808-10 PMID: 6191218
  16. The major histocompatibility complex-restricted antigen receptor on T cells. II. Role of the L3T4 product.
    J Exp Med. 1983 Oct 1;158(4):1077-91 PMID: 6413636
  17. The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors.
    Cell. 1983 Oct;34(3):717-26 PMID: 6605197
  18. Precursors of T cell growth factor producing cells in the thymus: ontogeny, frequency, and quantitative recovery in a subpopulation of phenotypically mature thymocytes defined by monoclonal antibody GK-1.5.
    J Exp Med. 1983 Nov 1;158(5):1654-71 PMID: 6195289
  19. Transient expression of interleukin 2 receptors. Consequences for T cell growth.
    J Exp Med. 1983 Dec 1;158(6):1895-911 PMID: 6606011
  20. Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1509-13 PMID: 6231642
  21. The proliferating cells in autoimmune MRL/lpr mice lack L3T4, an antigen on "helper" T cells that is involved in the response to class II major histocompatibility antigens.
    J Immunol. 1984 Jun;132(6):2686-9 PMID: 6144707
  22. Interleukin 2 regulates expression of its receptor and synthesis of gamma interferon by human T lymphocytes.
    Science. 1984 Jul 27;225(4660):429-30 PMID: 6429853
  23. Differentiation of thymocytes in fetal organ culture: analysis of phenotypic changes accompanying the appearance of cytolytic and interleukin 2-producing cells.
    J Immunol. 1984 Sep;133(3):1117-23 PMID: 6611365
  24. The heterogeneity and functional capacities of human thymocyte subpopulations.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4912-6 PMID: 6611554
  25. Interleukin 2 regulates the expression of Tac antigen on peripheral blood T lymphocytes.
    J Exp Med. 1984 Nov 1;160(5):1390-403 PMID: 6092510
  26. Genes encoding the Ti beta subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression.
    Cell. 1984 Dec;39(2 Pt 1):261-6 PMID: 6333925
  27. Clonotypic surface structure on human T lymphocytes: functional and biochemical analysis of the antigen receptor complex.
    Immunol Rev. 1984 Oct;81:95-129 PMID: 6334644
  28. Detection and localization by the monoclonal anti-interleukin 2 receptor antibody AMT-13 of IL 2 receptor-bearing cells in the developing thymus of the mouse embryo and in the thymus of cortisone-treated mice.
    Eur J Immunol. 1984 Dec;14(12):1152-6 PMID: 6440796
  29. Organ-specific autoimmune diseases induced in mice by elimination of T cell subset. I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause of autoimmune disease.
    J Exp Med. 1985 Jan 1;161(1):72-87 PMID: 3871469
  30. Ontogeny of the T-cell antigen receptor within the thymus.
    Nature. 1985 Feb 14-20;313(6003):592-5 PMID: 3871511
  31. Modulation of cytolytic T cell function by lectins.
    J Immunol. 1985 Apr;134(4):2179-84 PMID: 3871807
  32. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
    Eur J Immunol. 1979 Mar;9(3):205-10 PMID: 376318
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6268
Published
1985-09-00
Pages
6268-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391034
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.18.6268 PMC Full Text View journal details
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