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PMID: 6983073 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antigen-specific human T-cell hybridomas with helper activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 21 ·1982-11-00 ·Pages 6646-50

DeFreitas EC, Vella S, Linnenbach A, Zmijewski C, Koprowski H, Croce CM

Abstract

Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 after exposure to TeT on semiautologous monocytes and for their ability to bind to TeT-pulsed semiautologous monocytes. These antigen-specific T hybridomas demonstrated potent helper activity for semiautologous B cells as determined by the production of high levels of anti-TeT antibody in vitro.

MeSH Terms
Antibody Formation B-Lymphocytes/immunology Cell Line Humans Hybridomas/immunology Interleukin-2/biosynthesis Lymphocyte Cooperation Receptors, Antigen, T-Cell/immunology T-Lymphocytes/immunology Tetanus Toxoid/immunology
Chemicals
Interleukin-2 Receptors, Antigen, T-Cell Tetanus Toxoid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
DeFreitas E C
Vella S
Linnenbach A
Zmijewski C
Koprowski H
Croce C M
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.21.6646
Published
1982-11-00
Pages
6646-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347185
Subset
IM
Grants
NCI NIH HHS · CA 10815 · United States
NCI NIH HHS · CA 20741 · United States
NCI NIH HHS · CA 21069 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.21.6646 PMC Full Text View journal details
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