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PMID: 6972044 Published · ppublish English Journal Article

Antigen-specific human T-cell clones: development of clones requiring HLA-DR-compatible presenting cells for stimulation in presence of antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1858-62

Sredni B, Volkman D, Schwartz RH, Fauci AS

Abstract

A method for cloning soluble antigen-specific proliferating human T lymphocytes directly from peripheral blood cells of individuals recently primed with the antigen is described. The soluble antigen was keyhole limpet hemocyanin. After expansion in liquid culture, these cells were shown to be antigen specific and to require HLA-DR-histocompatible presenting cells. Recloning of small numbers of these cells in soft agar under conditions of high plating efficiency yielded true clones (i.e., derived from a single cell) which retained specificity for the original stimulating antigen as shown by blastogenic responses measured by incorporation of [3H]thymidine. However, antigen-specific stimulation of clones was demonstrated only in the presence of an HLA-DR-compatible cooperating cell population together with the relevant antigen. Cells that manifested only HLA-A or B locus identity with the T-cell clone were not effective as presenting cells. Other antigens, such as tetanus toxoid, to which the donor of the clone or of the presenting cells was immune failed to stimulate the clone.

MeSH Terms
Antigens, Surface/immunology Cell Division Cell Membrane/immunology Cells, Cultured Clone Cells HLA-DR Antigens Histocompatibility Antigens Class II/analysis Histocompatibility Testing Humans Rosette Formation T-Lymphocytes/immunology
Chemicals
Antigens, Surface HLA-DR Antigens Histocompatibility Antigens Class II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sredni B
Volkman D
Schwartz R H
Fauci A S
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1858
Published
1981-03-00
Pages
1858-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319234
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.3.1858 PMC Full Text View journal details
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