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PMID: 3932331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12.

Journal of bacteriology ·Vol. 164 ·No. 2 ·1985-11-00 ·Pages 836-44

Lloyd RG, Buckman C

Abstract

Evidence is presented to show that Escherichia coli JC7618, JC7621, and JC7623, previously regarded as having a recB recC sbcB genotype, carry an additional mutation in a new gene designated sbcC at minute 9 on the standard genetic map. In the absence of the sbcC mutation these strains are sensitive to mitomycin C and have a reduced efficiency of recombination. Cultures of recBC sbcB (sbcC+) strains grow slowly, contain many inviable cells, and rapidly accumulate fast-growing variants due to mutation of sbcC. sbcC has been identified on recombinant plasmids and tentatively located by Tn1000 mutagenesis to a 0.9-kilobase DNA section between proC and phoR.

MeSH Terms
Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Conjugation, Genetic Drug Resistance, Microbial Escherichia coli/drug effects,genetics,growth & development Genes, Bacterial Mitomycin Mitomycins/pharmacology Mutation Recombination, Genetic Suppression, Genetic Transduction, Genetic
Chemicals
Mitomycins Mitomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lloyd R G
Buckman C
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.2.836-844.1985
Published
1985-11-00
Pages
836-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214327
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.164.2.836-844.1985 PMC Full Text View journal details
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