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PMID: 6346018 Published · ppublish English Journal Article

Plasmidic recombination in Escherichia coli K-12: the role of recF gene function.

Molecular & general genetics : MGG ·Vol. 189 ·No. 3 ·1983-00-00 ·Pages 471-4

Cohen A, Laban A

Abstract

Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene products (exonuclease V and exonuclease I) in plasmidic recombination in wild-type E. coli cells and in cells in which the recE recombination pathway is activated. RecF gene function is required for interplasmidic recombination regardless of the recB recC genotype. Intraplasmidic recombination is recF dependent in cells having a functional exonuclease V, but not in recB recC mutants. Exonuclease V activity inhibits both interplasmidic and intraplasmidic recombination via the recE pathway.

MeSH Terms
Bacterial Proteins/physiology Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/physiology Genes, Bacterial R Factors Rec A Recombinases Recombination, Genetic
Chemicals
Bacterial Proteins Escherichia coli Proteins Rec A Recombinases Exodeoxyribonucleases recE protein, E coli exodeoxyribonuclease I Exodeoxyribonuclease V exodeoxyribonuclease V, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cohen A
Laban A
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00325911
Published
1983-00-00
Pages
471-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00325911
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