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PMID: 3932365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Retention of membrane proteins by the endoplasmic reticulum.

The Journal of cell biology ·Vol. 101 ·No. 5 Pt 1 ·1985-11-00 ·Pages 1724-32

Brands R, Snider MD, Hino Y, Park SS, Gelboin HV, Rothman JE

Abstract

We have used a monoclonal antibody specific for a hydrocarbon-induced cytochrome P450 to localize, by electron microscopy, the epitope-specific cytochrome P450. The cytochrome was found in the rough and smooth endoplasmic reticulum (ER) and the nuclear envelope of hepatocytes. Significant quantities of cytochrome P450 were not found in Golgi stacks. We also could not find any evidence of Golgi-associated processing of the Asn-linked oligosaccharide chains of two well-characterized ER membrane glycoprotein enzymes (glucosidase II and hexose-6-phosphate dehydrogenase), or of the oligosaccharides attached to the bulk of the glycoproteins of the ER membrane. We conclude that these ER membrane proteins are efficiently retained during a process of highly selective export from this organelle.

MeSH Terms
Acetylglucosaminidase/analysis Animals Concanavalin A/metabolism Cytochrome P-450 Enzyme System/analysis Endoplasmic Reticulum/metabolism,ultrastructure Glucosephosphate Dehydrogenase/analysis Golgi Apparatus/metabolism,ultrastructure Liver/metabolism,ultrastructure Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Membrane Proteins/metabolism Microscopy, Electron Oligosaccharides/analysis Rats Rats, Inbred Strains Receptors, Concanavalin A/metabolism
Chemicals
Membrane Proteins Oligosaccharides Receptors, Concanavalin A Concanavalin A Cytochrome P-450 Enzyme System Glucosephosphate Dehydrogenase Acetylglucosaminidase Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brands R
Snider M D
Hino Y
Park S S
Gelboin H V
Rothman J E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.5.1724
Published
1985-11-00
Pages
1724-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113977
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.5.1724 PMC Full Text View journal details
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