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PMID: 3934668 Published · ppublish English Journal Article

Activators of protein kinase C down-regulate and phosphorylate the T3/T-cell antigen receptor complex of human T lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 8158-62

Cantrell DA, Davies AA, Crumpton MJ

Abstract

As judged by indirect immunofluorescence, phorbol 12,13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using 125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr 26,000 gamma chain and to a lesser extent of the Mr 21,000 delta chain. No phosphorylation of the T3 epsilon chain or of the alpha and beta subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 gamma and delta chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.

MeSH Terms
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface Cell Compartmentation/drug effects Cell Membrane/metabolism Diglycerides/pharmacology Enzyme Activation/drug effects Humans Macromolecular Substances Phorbol 12,13-Dibutyrate Phorbol Esters/pharmacology Phosphorylation Protein Kinase C/physiology Receptors, Antigen, T-Cell/metabolism T-Lymphocytes/metabolism
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface Diglycerides Macromolecular Substances Phorbol Esters Receptors, Antigen, T-Cell Phorbol 12,13-Dibutyrate Protein Kinase C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cantrell D A
Davies A A
Crumpton M J
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.8158
Published
1985-12-00
Pages
8158-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391462
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.23.8158 PMC Full Text View journal details
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