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PMID: 6601175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody.

The Journal of experimental medicine ·Vol. 157 ·No. 4 ·1983-04-01 ·Pages 1149-69

Haskins K, Kubo R, White J, Pigeon M, Kappler J, Marrack P

Abstract

An antibody-secreting B cell hybridoma, KJ1-26.1, has been prepared from mice immunized with the T cell hybridoma DO-11.10, which recognizes chicken ovalbumin in association with I-Ad (cOVA/I-Ad). KJ1-26.1 blocks I-restricted antigen recognition by DO-11.10 and a subclone of this T cell hybridoma, DO-11.10.24, which has the same specificity for cOVA/I-Ad as its parent. KJ1-26.1 does not block I-restricted antigen recognition by any other T cell hybridoma tested, including a number of T cell hybridomas closely related to DO-11.10, with similar, but not identical, specificities for antigen/I. Moreover, KJ1-26.1 binds to DO-11.10 and DO-11.10.24, but not to any other T cell hybridomas tested, including three subclones of DO-11.10 that have lost the ability to recognize cOVA/I-Ad. Thus, in every regard KJ1-26.1 appears to be binding to all or part of the receptors for antigen/I on the T cell hybridoma DO-11.10. KJ1-26.1 appears to bind to approximately 15,000 molecules/cell on the surface of DO-11.10. The antibody precipitates an 80,000 dimer from the cells, which on reduction migrates as 40-44,000 monomers. The receptor(s) for antigen/I on DO-11.10 therefore includes molecules with these properties.

MeSH Terms
Animals Antibodies, Monoclonal/analysis,biosynthesis,immunology Antibody Specificity Antigen-Antibody Reactions Binding Sites, Antibody Chemical Precipitation Electrophoresis, Polyacrylamide Gel Histocompatibility Antigens/genetics,immunology Hybridomas/immunology Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Molecular Weight Receptors, Antigen, T-Cell/genetics,immunology,isolation & purification T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Histocompatibility Antigens Receptors, Antigen, T-Cell
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Haskins K
Kubo R
White J
Pigeon M
Kappler J
Marrack P
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54 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.157.4.1149
Published
1983-04-01
Pages
1149-69
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186983
Subset
IM
Grants
NIAID NIH HHS · AI-07035 · United States
NIAID NIH HHS · AI-12136 · United States
NIAID NIH HHS · AI-18785 · United States
External Links
PubMed source DOI: 10.1084/jem.157.4.1149 PMC Full Text View journal details
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