Subunit structure of cell surface proteins: disulfide bonding in antigen receptors, Ly-2/3 antigens, and transferrin receptors of murine T and B lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4530-4

Goding JW, Harris AW

Abstract

The surface proteins of lymphocytes from spleen and thymus and several cultured lymphoid tumor lines were radioiodinated in situ, solubilized with Triton X-100, and examined for the presence of disulfide-bonded subunits by two-dimensional (intact, reduced) NaDodSO4/polyacrylamide gel electrophoresis. [Hynes, R. O. & Destree, A. (1977) Proc. Natl. Acad. Sci. USA 74, 2855-2859]. Few lymphocyte surface proteins were found to consist of disulfide-bonded subunits, and the most prominent of these could be identified. In normal B lymphocytes and B-lymphoma cells, IgD or IgM (or both) were the major disulfide-bonded proteins, and these were easily detectable, even without immunoprecipitation. In contrast, analysis of thymocytes and T-lymphoma cells did not reveal any protein resembling immunoglobulin in its chain structure. The major labeled thymocyte membrane protein consisting of disulfide-bonded subunits was identified as the Ly-2/3 antigen. It appeared to contain disulfide-bonded homodimers of Mr 35,000 (alpha 2) noncovalently associated with a second pair of homodimers of Mr 30,000 (beta 2). Peptide mapping showed these polypeptides to be homologous. A third disulfide-bonded homodimer, which was heterogeneous in apparent Mr, appeared to be part of the Ly-2/3 complex. All cultured T- and B-lymphoma lines examined were found to possess a major surface protein that appeared to be a disulfide-bonded homodimer of a polypeptide of Mr 95,000. This protein was identified as the receptor for transferrin. It is suggested that the presence of two or more subunits in cell surface receptors renders their ligand functionally bivalent, making ligand-induced receptor aggregation possible.

MeSH Terms

Animals Antigens, Ly B-Lymphocytes/ultrastructure Cell Line Cell Membrane/ultrastructure Disulfides Immunoglobulin D Immunoglobulin M Macromolecular Substances Membrane Proteins Mice Receptors, Antigen Receptors, Cell Surface Receptors, Transferrin T-Lymphocytes/ultrastructure

Chemicals

Antigens, Ly Disulfides Immunoglobulin D Immunoglobulin M Macromolecular Substances Membrane Proteins Receptors, Antigen Receptors, Cell Surface Receptors, Transferrin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goding J W

Harris A W

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31 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.7.4530

Published

1981-07-00

Pages

4530-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319825

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.7.4530 PMC Full Text View journal details

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Subunit structure of cell surface proteins: disulfide bonding in antigen receptors, Ly-2/3 antigens, and transferrin receptors of murine T and B lymphocytes.

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