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PMID: 3951999 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and nucleotide sequence of the streptavidin gene.

Nucleic acids research ·Vol. 14 ·No. 4 ·1986-02-25 ·Pages 1871-82

Argaraña CE, Kuntz ID, Birken S, Axel R, Cantor CR

Abstract

Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3' and 5' flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.

MeSH Terms
Amino Acid Sequence Avidin Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Genes, Bacterial Protein Conformation Streptavidin Streptomyces/genetics
Chemicals
Bacterial Proteins DNA, Bacterial Avidin Streptavidin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Argaraña C E
Kuntz I D
Birken S
Axel R
Cantor C R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.4.1871
Published
1986-02-25
Pages
1871-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339579
Subset
IM
Grants
NCI NIH HHS · CA39782 · United States
NIGMS NIH HHS · GM14825 · United States
NICHD NIH HHS · HD15454 · United States
Databases
GENBANK
X03591
External Links
PubMed source DOI: 10.1093/nar/14.4.1871 PMC Full Text View journal details
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