Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. II. Biochemistry.

Molecular & general genetics : MGG ·Vol. 177 ·No. 1 ·1979-00-00 ·Pages 47-56

Sor F, Faye G

Abstract

1. Several nuclear mutants have been isolated which showed thermo- or cryo-sensitive growth on non-fermentable media. Although the original strain carried mitochondrial drug resistance mutations (CR, ER, OR and PR), the resistance to one or several drugs was suppressed in these mutants. Two of them showed a much reduced amount of the mitochondrial small ribosomal subunit (37S) and of the corresponding 16S ribosomal RNA. Two dimensional electrophoretic analysis did not reveal any change in the position of any of the mitochondrial ribosomal proteins. However one of the mitochondrial ribosomal proteins. However one of the mutants showed a striking decrease in the amounts of three ribosomal proteins S3, S4 and S15. 2. Four temperature-sensitive mitochondrial mutations have been localized in the region of the gene coding for the large mitochondrial ribosomal RNA (23S). These mutants all showed a marked anomaly in the mitochondrial large ribosomal subunit (50S) and/or the corresponding 23S ribosomal RNA.

MeSH Terms

Cell Nucleus/metabolism Chromosome Mapping DNA, Mitochondrial/metabolism Drug Resistance Mutation RNA, Fungal/analysis,genetics RNA, Ribosomal/analysis,genetics Ribosomal Proteins/genetics Ribosomes/metabolism Saccharomyces cerevisiae/genetics,ultrastructure Temperature

Chemicals

DNA, Mitochondrial RNA, Fungal RNA, Ribosomal Ribosomal Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sor F

Faye G

References (20)

20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267252

Published

1979-00-00

Pages

47-56

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267252

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Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. II. Biochemistry.

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