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PMID: 3990684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Abundant nuclear ribonucleoprotein form of CAD RNA.

Molecular and cellular biology ·Vol. 5 ·No. 3 ·1985-03-00 ·Pages 569-75

Sperling R, Sperling J, Levine AD, Spann P, Stark GR, Kornberg RD

Abstract

Transcripts of the CAD gene in Syrian hamster cells are as abundant in the nucleus as in the cytoplasm. This was shown by in situ hybridization of whole cells and by solution and blot hybridization of subcellular fractions. Similar results were obtained both for wild-type cells and for a mutant containing amplified CAD genes in which the level of CAD RNA is 150-fold greater. CAD nuclear RNA is indistinguishable from mature mRNA by gel electrophoresis and blot hybridization. Discrete higher-molecular-weight precursors are undetectable, although the persistence of a short length of intervening sequence in the otherwise fully processed RNA is not excluded. CAD RNA is released from nuclei by sonication in physiological conditions in a ribonucleoprotein form that sediments as a broad peak at about 200S in a sucrose gradient. CAD sequences extracted from nuclei by treatment with EDTA and RNase are found in the 30S particles previously described.

MeSH Terms
Animals Aspartate Carbamoyltransferase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Cell Nucleus/analysis Cloning, Molecular Cricetinae Cytoplasm/analysis Dihydroorotase Gene Amplification Mesocricetus/genetics Multienzyme Complexes/genetics Proteins/genetics RNA Splicing RNA, Messenger/analysis Ribonucleoproteins/analysis
Chemicals
CAD trifunctional enzyme Multienzyme Complexes Proteins RNA, Messenger Ribonucleoproteins Aspartate Carbamoyltransferase Dihydroorotase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sperling R
Sperling J
Levine A D
Spann P
Stark G R
Kornberg R D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.3.569-575.1985
Published
1985-03-00
Pages
569-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366750
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.3.569-575.1985 PMC Full Text View journal details
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